Effects of Creatine and Its Analog, β-Guanidinopropionic Acid, on the Differentiation of and Nucleoli in Myoblasts
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概要
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The effects of supplementation with creatine (Cr) and its analog, β-guanidinopropionic acid (β-GPA), on the differentiation of myoblasts and the numbers of nucleoli were studied in C2C12 cells. The cells were cultured in differentiation medium for 4 d. Then Cr (1 mM) or β-GPA (1 mM) was added to the cells, and the mixture was cultured for an additional 2 d. Although the number of myotubes was not different among the groups, myotube diameters and nuclear numbers in myotubes were increased by Cr and β-GPA treatment respectively. The expression of differentiation marker proteins, myogenin, and the myosine heavy chain, was increased in the β-GPA group. Supplementation with β-GPA also increased the percentage of p21 (inhibitor for cell cycle progression)-positive myoblasts. Supplementation with Cr inhibited the decrease in nucleoli numbers, whereas β-GPA increased nucleolar sizes in the myotubes. These results suggest that β-GPA supplementation stimulated the differentiation of myoblasts into multi-nucleated myotubes through induction of p21 expression.
著者
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Nakai Naoya
Department Of Applied Photonics System Technology Chitose Institute Of Science And Technology
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Kawano Fuminori
Section Of Applied Physiology Department Of Health And Sport Sciences Graduate School Of Medicine Os
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Ogura Akihiko
Department Of Biology Faculty Of Science Graduate School Of Osaka University
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Ohira Yoshinobu
Department Of Kinesiology University Of California
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Okumura Yuushi
Graduate School Of Medicine Osaka University
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Ohira Takashi
Department Of Information And Computer Sciences Toyohashi University Of Technology
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KAWANO Fuminori
Department of Physiology and Biomechanics, NIFS-K
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Terada Masahiro
Department Of Chemical Technology Tokyo Institute Of Technology
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Matsuoka Yoshikazu
Department Of Stem Cell Biology And Regenerative Medicine Graduate School Of Medical Science Kansai
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HIGO Yoko
Department of Health and Sports Sciences, Graduate School of Frontier Biosciences, Osaka University
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OKE Yoshihiko
Department of Health and Sports Sciences, Graduate School of Medicine, Osaka University
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Kawano Fuminori
Department Of Health And Sports Sciences Graduate School Of Medicine Osaka University
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