Inhibitory Effect of Bovine Lactoferrin on Human Parainfluenza Virus Type 2 Infection
スポンサーリンク
概要
- 論文の詳細を見る
Lactoferrin (Lf) is a multifunctional protein that has inhibitory activity against microorganisms. In this study, the effects of Lf on the growth of human parainfluenza virus type 2 (hPIV-2) in LLCMK2 cells were investigated. Lf inhibited cell fusion and hemadsorption induced by hPIV-2. However, virus RNA synthesis was only slightly inhibited by Lf. In addition, indirect immunofluorescence study showed that virus protein syntheses were not completely inhibited by Lf. Using a recombinant, green fluorescence protein-expressing hPIV-2 (rghPIV-2), it was found that virus entry into cells were considerably inhibited by Lf, but cell-to-cell spread was not inhibited. The number of viruses produced by the cells were determined, and it was found that Lf reduced the number of released viruses to about 1/300 compared with that of positive control. Lf bound to cell surface within 10 min at early phase of infection, it assembled gradually, and many aggregates were observed at 120 min. These results indicated that Lf considerably inhibited virus adsorption to the surface of the cells by binding to the cell surface and prevented hPIV-2 infection.
著者
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Yamamoto Hidetaka
Department Of Anatomic Pathology Graduate School Of Medical Sciences Kyushu University
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Yamamoto Hidetaka
Department Of Microbiology Suzuka University Of Medical Science
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Yamamoto Hidetaka
Department Of Anatomic Pathology Faculty Of Medicine Kyushu University
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Ura Yukari
Department of Microbiology, Suzuka University of Medical Science
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Tanemura Miho
Department of Microbiology, Suzuka University of Medical Science
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Koyama Aoi
Department of Microbiology, Suzuka University of Medical Science
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Takano Sayaka
Department of Microbiology, Suzuka University of Medical Science
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Uematsu Jun
Department of Microbiology, Suzuka University of Medical Science
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Kawano Mitsuo
Department of Microbiology, Mie University Graduate School of Medicine
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Tsurudome Masato
Department of Microbiology, Mie University Graduate School of Medicine
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OBrien Myles
Graduate School of Mie Prefectural College of Nursing
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Komada Hiroshi
Department of Microbiology, Graduate School of Clinical Nutrition, Suzuka University of Medical Scie
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Komada Hiroshi
Department Of Biology Suzuka University Of Medical Science And Technology
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