イヌプロラクチンの分子クローニングと発現

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概要

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• We isolated canine prolactin cDNA from a dog pituitary cDNA library. The 930-bp nucleotide sequence covered the entire open reading frame encoding the putative 229 amino acids. It was located in chromosome 35, and had five exons. The amino acid sequence was highly homologous to the feline and porcine sequences. To generate recombinant canine prolactin, plasmids for full-length canine prolactin were constructed and transfected into the mammalian HEK293 cell line. The recombinant prolactin was secreted into the medium as an N-linked glycosylated (31 kDa) or non-glycosylated (27 kDa) protein. Western blotting revealed both of these bands were canine pituitary protein extracts.

著者

• Hasegawa Yoshihisa

  Laboratory Animal Science Kitasato University School Of Veterinary Medicine And Animal Science

• Kawaminami Mitsumori

  Laboratory Of Veterinary Physiology Kitasato University

• KAWAMINAMI Mitsumori

  Laboratory of Veterinary Physiology, Kitasato University, School of Veterinary Medicine

• 橋本 統

  Laboratory of Experimental Animal Science, Kitasato University, School of Veterinary Medicine

• 米澤 智洋

  Laboratory of Veterinary Physiology, Kitasato University, School of Veterinary Medicine

• 杉山 由夏

  Laboratory of Experimental Animal Science, Kitasato University, School of Veterinary Medicine

• HASEGAWA Yoshihisa

  Laboratory of Experimental Animal Science, Kitasato University, School of Veterinary Medicine

• SUGIYAMA Yuka

  Laboratory of Experimental Animal Science, Kitasato University, School of Veterinary Medicine

• KAWAMINAMI Mitsumori

  Laboratory of Physiology, Kitasato University School of Veterinary Medicine and Animal Sciences

関連論文

• Analysis of the Function of Activin β_C Subunit Using Recombinant Protein
• Immunohistochemical Localization of Annexin A5 in the Mammary Gland of Rats: Up-Regulation of the Expression by Pup Removal
• Stimulation of Annexin A5 Expression by Gonadotropin Releasing Hormone (GnRH) in the Leydig Cells of Rats
• Annexin 5 Inhibits Thyrotropin Releasing Hormone (TRH) Stimulated Prolactin Release in the Primary Culture of Rat Anterior Pituitary Cells
• イヌプロラクチンの分子クローニングと発現
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