Flk1-GFP BAC Tg Mice: An Animal Model for the Study of Blood Vessel Development
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概要
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The mouse Flk1 (also called Kdr or Vegf-r2) gene encodes a receptor for VEGF-A. Flk1 is expressed in endothelial cells of the developing embryo. Recent studies have shown that Flk1 is expressed by multi-potent mesodermal progenitors, which give rise to various hematopoietic and cardiovascular cell lineages during development, and in differentiating ES cells, which may be used for cell transplantation therapy to treat cardiovascular diseases. Given its developmental and clinical importance in cardiovascular tissues, an animal model of Flk1 activity would be very useful. Here, we report the generation of Flk1-GFP BAC transgenic mice for monitoring Flk1 gene expression during development. We show that GFP expression in these mice serves as a surrogate marker for developing endothelial cells. Immunohistochemical analysis showed that the regions of expression of GFP and endogenous FLK1 largely overlap. Uniform GFP expression was observed in most endothelial cells at 8.5 dpc and thereafter. Flk1-GFP BAC transgenic mice should be useful for the study of both vascular development and pathological angiogenesis.
著者
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TAKAHASHI Satoru
Department of Experimental Pathology and Tumor Biology, Nagoya City University Graduate School of Me
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ITOH SUSUMU
Department of Pediatrics, Kagawa University
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Itoh Fumiko
Department Of Experimental Pathology Graduate School Of Comprehensive Human Sciences University Of T
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Ema Masatsugu
Department Of Biomolecular Sciences Graduate School Of Life Science Tohoku University
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Matsumoto Ken
Department Of Oral And Maxillofacial Surgery Ii Osaka University Faculty Of Dentistry
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Itoh Susumu
Department Of Experimental Pathology Graduate School Of Comprehensive Human Sciences University Of T
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ISHITOBI Hiroyuki
Department of Anatomy and Embryology, Institute of Basic Medical Sciences, Graduate School of Compre
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AZAMI Takuya
Department of Anatomy and Embryology, Institute of Basic Medical Sciences, Graduate School of Compre
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Takahashi Satoru
Department Of Anatomy And Embryology Biomolecular And Integrated Medical Sciences Graduate School Of
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Itoh Susumu
Department Of Electrical Engineering Faculty Of Science And Technology Science University Of Tokyo
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Matsumoto Ken
Department Of Biology Faculty Of Science Chiba University
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ISHITOBI Hiroyuki
Department of Anatomy and Embryology, Institute of Basic Medical Sciences, Graduate School of Comprehensive Human Sciences, University of Tsukuba
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石飛 博之
Department of Anatomy and Embryology, Institute of Basic Medical Sciences, Graduate School of Comprehensive Human Sciences, University of Tsukuba
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松本 健
Department of Anatomy and Embryology, Institute of Basic Medical Sciences, Graduate School of Comprehensive Human Sciences, University of Tsukuba
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浅見 拓哉
Department of Anatomy and Embryology, Institute of Basic Medical Sciences, Graduate School of Comprehensive Human Sciences, University of Tsukuba
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EMA Masatsugu
Department of Anatomy and Embryology, Institute of Basic Medical Sciences, Graduate School of Comprehensive Human Sciences, University of Tsukuba
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Itoh Susumu
Department of Experimental Pathology, Graduate School of Comprehensive Human Sciences, University of Tsukuba
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AZAMI Takuya
Department of Anatomy and Embryology, Institute of Basic Medical Sciences, Graduate School of Comprehensive Human Sciences, University of Tsukuba
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