Scavenging Effects of Metal Complexes of Water-Soluble Thiacalix[4]arenetetrasulfonate on Superoxide Anion Radicals
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概要
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The scavenging effects of metal complexes of thiacalix[4]arenetetrasulfonate (Me-TCAS[4], Me=H2, Fe3+, Mn3+, Mn2+, Cu2+, and Zn2+) on superoxide anion radicals (O2−) generated from the xanthine–xanthine oxidase system were investigated by the nitroblue tetrazolium (NBT) method and electron spin resonance (ESR) spin-trapping method using 5,5-dimethyl-1-pyrroline-N-oxide as a trapping reagent. As a reference, calix[4]arenetetrasulfonate (H2-CAS[4]), calix[6]arenehexasulfonate (H2-CAS[6]) and calix[8]areneoctasulfonate (H2-CAS[8]) were also examined. The results by the NBT method indicated that Fe3+- and Mn3+-TCAS[4] exhibited the highest O2− scavenging activity among Me-TCAS[4] and H2-CAS[n] (n=4, 6, 8) in this study. The IC50 values of Fe3+- and Mn3+-TCAS[4] for O2− scavenging activity were estimated to be 5.3 and 7.8 μM, respectively, and were almost the same as those of tannin acid, catechin and their derivatives, which are known as very effective scavengers of O2−. Scavenging activities were in the order of Fe3+- and Mn3+-TCAS[4]>>Mn2+-, Cu2+-, and Zn2+-TCAS[4]>>H2-TCAS[4] and H2-CAS[n] (n=4, 6, 8). Each activity of Me-TCAS[4] (Me=Fe3+, Mn3+, Mn2+, Cu2+, and Zn2+) was higher than that of the corresponding metal ion, indicating that H2-TCAS[4] has the ability to raise the activity of the metal ion itself by forming a complex. Also, the ESR spin-trapping method revealed that Fe3+- and Mn3+-TCAS[4] showed high O2− scavenging activities, similarly to the results by the NBT method.
- 公益社団法人 日本薬学会の論文
著者
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Odo Junichi
Department Of Biological Chemistry Faculty Of Science Okayama University Of Science
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YOSHIDA AKIKO
Department of Anesthesiology, Tohoku University Hospital
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OHNISHI YUKI
Department of Anesthesiology, School of Medicine, Keio University
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Inoguchi Masahiko
Department of Biological Chemistry, Faculty of Science, Okayama University of Science
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Inoguchi Masahiko
Department Of Biochemistry Okayama University Of Science
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Hayashi Ken-ichiro
Department of Agricultural Chemistry, Faculty of Agriculture, University of Osaka Prefecture
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Ohnishi Yuki
Department Of Anesthesiology School Of Medicine Keio University
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Kimura Ayumi
Department of Biological Chemistry, Faculty of Science, Okayama University of Science
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Matsu-ura Atsushi
Department of Biological Chemistry, Faculty of Science, Okayama University of Science
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Saeki Yoshiro
Department of Biological Chemistry, Faculty of Science, Okayama University of Science
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Ueki Takahiko
Department of Biological Chemistry, Faculty of Science, Okayama University of Science
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Yoshida Akiko
Department Of Anesthesiology Tohoku University Hospital
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YOSHIDA Akiko
Department of Anesthesiology and Perioperative Medicine, Tohoku University Postgraduate Medical School
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