Anti-adenovirus Activities of Shikonin, a Component of Chinese Herbal Medicine in Vitro

スポンサーリンク

概要

• 論文の詳細を見る

• Radix Lithosperm eyrthrorhizon is a common prescription compound in traditional Chinese medicine. Shikonin is a major component of Radix Lithospermi and has various biological activities. We have investigated the inhibitory effect of shikonin on the growth of adenovirus type 3 (AdV3) in vitro. The antiviral function of shikonin against AdV3 and its virus inhibition ratio were detected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide method (MTT). The expression of hexon protein in AdV3 was determined by immunofluorescence assay using laser scanning confocal microscopy (LSCM) and Western blot analysis. In addition, the rate of apoptosis in cells infected by AdV3 was determined by flow cytometry. Shikonin (0.0156—1 μM) inhibited growth of AdV3 in a concentration-dependent manner with a virus inhibition rate of 23.8—69.1%. Expression of hexon protein in AdV3 was higher in the virus control group than in the shikonin-treated groups as determined by immunofluorescence assay and Western blotting (p<0.05). The rate of shikonin-treated HeLa cell apoptosis had a statistically significant decrease with increasing concentration of drug (p<0.05). Our data demonstrate that shikonin possesses anti-AdV3 capabilities and that the potential antiviral mechanism might involve inhibiting the degree of apoptosis and hexon protein expression of AdV.

著者

• Gao Hong

  Department of Hygienic Microbiology, Public Health College, Harbin Medical University

• Liu Lei

  Department of Hygienic Microbiology, Public Health College, Harbin Medical University

• Qu Zhang-Yi

  Department of Hygienic Microbiology, Public Health College, Harbin Medical University

• Wei Feng-xiang

  Department of Hygienic Microbiology, Public Health College, Harbin Medical University

• Wang Shu-qiu

  College of Basic Medical Sciences, Jamusi University

• Chen Guang

  College of Basic Medical Sciences, Jamusi University

• Qin Le

  Department of Hygienic Microbiology, Public Health College, Harbin Medical University

• Jiang Fu-yang

  Department of Hygienic Microbiology, Public Health College, Harbin Medical University

• Wang Ying-chen

  Department of Hygienic Microbiology, Public Health College, Harbin Medical University

• Shang Lei

  Department of Hygienic Microbiology, Public Health College, Harbin Medical University

• Gao and

  The Daqing Branch, Harbin Medical University

• Liu Lei

  Department Of Chemistry And State Key Laboratory Of Elemento-organic Chemistry Nankai University

• Gao Hong

  Department Of Energy And Environmental Science Graduate School Of Engineering Utsunomiya University

• Gao Chun-yan

  The Daqing Branch, Harbin Medical University

• LIU Lei

  Department of Chemistry and Chemical Engineering, Beijing University of Technology

関連論文

• Anti-adenovirus Activities of Shikonin, a Component of Chinese Herbal Medicine in Vitro
• Three Minor Novel Triterpenoids from the Leaves of Diospyros kaki
• Facile Derivatization of Pyridyloxazole-type Fluorophore via Click Chemistry
• Microbial Conversion of Rare Ginsenoside Rf to 20(S)-Protopanaxatriol by Aspergillus niger
• Enlerged Frequency Upshift from DC to AC Radiation Converter using Boundary Effect of Plasma-filled Waveguide
• Further Frequency Upshift in DC to AC Radiation Converter by Perpendicular DC Magnetic Field
• Blue-shifted Hydrogen Bonds with Proton-donors Incapable of Rehybridization
• Steric Effect is an Additional Possible Cause of Blue-shifting Hydrogen Bonds
• A Theoretical Study of the Substituent Effects on the P-X (X=H, F, Cl) Bond Dissociation Energies in para- and meta-Substituted Aromatic Phosphines
• Enthalpy-Entropy Compensation of Cyclodextrin Complexation with Different Classes of Substrates
• The Performance of the Benesi-Hildebrand Method in Measuring the Binding Constants of the Cyclodextrin Complexation
• Ipsilateral basicervical femoral neck and shaft fractures treated with long proximal femoral nail antirotation or various plate combinations : comparative study
• Microbial Conversion of Rare Ginsenoside Rf to 20(S)-Protopanaxatriol by Aspergillus niger
• Flavonoid Constituents from Spiranthes australis LINDL
• Tracking and Measuring a Moving Object Based on Multi-Sensory Information with Eye-in-Hand Robot System
• An Application of Visual Feedback Principle to Feature Extraction of Objects with Laser Range Finder
• A New Class of Acetophenone-based Cinchona Alkaloids as Phase-transfer Catalysts : Application to the Enantioselective Synthesis of α-Amino Acids
• Analysis of acetylcholine from extracellular fluid in brain by in vivo microdialysis and LC-ESI-MS/MS with the stable isotope-labeled internal standard
• Effects of H_2S on Myogenic Responses in Rat Cerebral Arterioles
• Enlarged Frequency Upshift from DC to AC Radiation Converter using Boundary Effect of Plasma-filled Waveguide
• Effect of Temperature on DNA Chromatographic Separation in Free Solution
• Isotopic Effect on Stereodynamics of the Reactions H + NeH^+/H + NeD^+/H + NeT^+

もっと見る 閉じる

スポンサーリンク