Microbial Conversion of Rare Ginsenoside Rf to 20(S)-Protopanaxatriol by Aspergillus niger
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概要
- 論文の詳細を見る
In this study, rare ginsenoside Rf was transformed into 20(S)-protopanaxatriol (PPT(S)) by glycosidase from Aspergillus niger. By investing the reaction conditions, the optimal conditions were obtained, as follows: pH 5.0, temperature 55 °C, and substrate concentration 1.25 mmol/l. Under optimal conditions, PPT(S) (1.13 μmol) prepared from 1.25 μmol Rf showed a higher yield (90.4%). The enzymatic reaction was analyzed by reversed-phase HPLC, suggesting the transformation pathway: Rf→Rh1(S)→PPT(S).
著者
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Liu Lei
Department of Hygienic Microbiology, Public Health College, Harbin Medical University
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Li Zheng
Department Of Developmental Genetics Kumamoto University
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WANG Zhen
Department of Pathology, Affiliated Tumor Hospital of Xinjiang Medical University
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GU Li-Juan
Department of Food Science and Technology, College of Agriculture and Biotechnology, Chungnam Nation
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ZHANG Dong-Liang
Department of Food Science and Technology, College of Agriculture and Biotechnology, Chungnam Nation
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WANG Chun-Yan
Department of Food Science and Technology, College of Agriculture and Biotechnology, Chungnam Nation
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SUNG Chang-Keun
Department of Food Science and Technology, College of Agriculture and Biotechnology, Chungnam Nation
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