変異原性試験による廃棄物埋立処分場浸出水中の微量汚染物質サーベイランス

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Mutagenic activity was examined on dichloromethane extracts of leachate samples collected from a municipal solid waste landfill by means of the Ames Salmonella assay. Mutagenicity was found among 51% of 101 leachate samples and most of the positive samples showed mutagenicity for both strains TA100 and TA98 irrespective of metabolic activation by S9, indicating that the active ingredients consisted mainly of direct mutagens. A four-year survey revealed that mutagenic activity in landfill leachate was high (200-1,500 net revertants·l-1) during the operation of the landfill, whereas it decreased gradually after the landfill was closed. Detailed study on clesed landfill sites showed that younger landfill sites discharged leachate with higher mutagenic activity. No correlation was observed between mutagenic activity and conventional water pollution indicators such as BOD, COD, TOC, DO, and water temperature except for T-N which showed a weak positive correlation. These results demonstrated the significance of the mutagenicity assay for evaluating the possible presence of micropollutants in landfill leachate.
社団法人　日本水環境学会の論文