Nuclear Factor-κB/Signal Transducers and Activators of Transcription-1-Mediated Inflammatory Responses in Lipopolysaccharide-Activated Macrophages Are a Major Inhibitory Target of Kahweol, a Coffee Diterpene

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概要

• 論文の詳細を見る

• Diterpene kahweol, one of the major components in coffee, has anti-cancer, anti-oxidative, and anti-inflammatory activity. In this study, we explored the molecular mechanism of the anti-inflammatory activity of kahweol. Lipopolysaccharide (LPS)-activated RAW264.7 cells were used to explore the modulatory role of kahweol on nitric oxide (NO) and prostaglandin E2 (PGE2) production and the activation of signaling proteins and transcription factors using immunoblotting and reverse transcription-polymerase chain reaction (RT-PCR). Kahweol diminished both the production of NO and PGE2 and the mRNA expression of inducible NO synthase (iNOS) and cyclooxygenase (COX)-2. Interestingly, this compound suppressed the phospho-signal transducers and activators of transcription (STAT)-1 and p65/nuclear factor (NF)-κB levels in the nucleus but not c-Jun and c-fos. In conjunction, the phosphorylation of Akt and Janus kinase (JAK)2 also decreased. Therefore, our data suggest that kahweol in coffee may be an anti-inflammatory modulator with NF-κB/STAT-1-targeted inhibitory properties in LPS-activated RAW264.7 cells.

• 日本薬学会の論文

著者

• Cho Jae

  School Of Bioscience And Biotechnology And Inst. Of Bioscience And Biotechnology Kangwon National Un

• Lee Jaehwi

  College of Pharmacy, Chung-Ang University

• Shen Ting

  School of Bioscience and Biotechnology, Kangwon National University

• Park Yung

  College of Forest and Environmental Sciences, Kangwon National University

• Kim Seong

  Laboratory of Chemical Genomics, Korea Research Institute of Chemical Technology

• Cho Jae

  School of Bioscience and Biotechnology, Kangwon National University

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