Function and Expression of ATP-Binding Cassette Transporters in Cultured Human Y79 Retinoblastoma Cells
スポンサーリンク
概要
- 論文の詳細を見る
The aim of this study was to reveal the expression and function of P-glycoprotein and multidrug resistance-associated proteins (MRP), members of the ATP-binding cassette (ABC) superfamily of drug transporters, in cultured human Y79 retinoblastoma cells. ABC transporter mRNA expression was evaluated by conventional reverse transcription-polymerase chain reaction (RT-PCR) and real-time PCR analyses. Cellular accumulation of rhodamine 123 (P-glycoprotein substrate), calcein (MRP substrate), and doxorubicin (P-glycoprotein/MRP substrate) was analyzed by fluorometry. Conventional RT-PCR analysis showed the expression of multidrug resistance 1 (MDR1), MRP1, MRP2 and lung resistance-related protein (LRP) mRNAs. Real-time RT-PCR analysis revealed that the expression levels of the MDR1 and MRP2 genes in Y79 cells were much lower than those in human intestinal cell line Caco-2, while the expression level of MRP1 was higher than that in Caco-2 cells. The accumulation of rhodamine 123 was not enhanced by verapamil or reversin 205, inhibitors of P-glycoprotein, indicating no function of P-glycoprotein in Y79 cells. The accumulation of calcein was significantly increased by various MRP inhibitors including probenecid, indicating that MRP functions in Y79 cells. The accumulation of doxorubicin was increased in the presence of metabolic inhibitors (10 mM 2-deoxyglucose and 5 mM sodium azide). However, most MRP inhibitors such as probenecid and indomethacin did not affect doxorubicin accumulation, while cyclosporin A and taclorimus significantly increased doxorubicin accumulation. These results suggest that MRP, but not P-glycoprotein, functions in Y79 cells, and that the efflux of doxorubicin from Y79 cells may be due to an ATP-dependent transporter, which has not been identified yet.
- 日本薬学会の論文
著者
-
SATO Koya
Department of Medicine and Science in Sports and Exercise, Tohoku University Graduate School of Medi
-
Sato Koya
Department Of Medicine And Science In Sports And Exercise Tohoku University Graduate School Of Medic
-
YUMOTO Ryoko
Department of Pharmaceutics and Therapeutics, Division of Clinical Pharmaceutical Science, Programs
-
NAGAI Junya
Department of Pharmaceutics and Therapeutics, Division of Clinical Pharmaceutical Science, Programs
-
TAKANO Mikihisa
Department of Pharmaceutics and Therapeutics, Division of Clinical Pharmaceutical Science, Programs
-
Ishikawa Yuka
Department of Pharmaceutics and Therapeutics, Graduate School of Biomedical Sciences, Hiroshima Univ
-
Okada Yumi
Department of Pharmaceutics and Therapeutics, Graduate School of Biomedical Sciences, Hiroshima Univ
-
Okada Yumi
Department Of Internal Medicine Miki Sanyo Hospital
-
Ishikawa Yuka
Department Of Pediatrics National Yakumo Hospital
-
Yumoto Ryoko
Department Of Pharmaceutics And Therapeutics Graduate School Of Biomedical Sciences Hiroshima Univer
-
Takano Mikihisa
Department Of Pharmaceutics And Therapeutics Graduate School Of Biomedical Sciences Hiroshima Univer
-
Nagai Junya
Department Of Pharmaceutics And Therapeutics Graduate School Of Biomedical Sciences Hiroshima Univer
-
TAKANO Mikihisa
Department of Pharmaceutics and Therapeutics, Graduate School of Biomedical Sciences, Hiroshima University
-
Okada Yumi
Department of Pharmaceutics and Therapeutics, Graduate School of Biomedical Sciences, Hiroshima University
-
Sato Koya
Department of Pharmaceutics and Therapeutics, Graduate School of Biomedical Sciences, Hiroshima University
関連論文
- Effects of Exercise Training on Physical Activity in Older People : a Randomized Controlled Trial
- Interaction of Endogenous Compounds in Human and Rat Urine with P-glycoprotein
- パクリタキセル耐性 HepG2 細胞の樹立と耐性化メカニズムの解析
- Function and Expression of ATP-Binding Cassette Transporters in Cultured Human Y79 Retinoblastoma Cells
- Transport Characteristics of Multidrug Resistance-associated Protein 5 in Human Erythrocyte Membranes
- Low-Affinity Transport of FITC-Albumin in Alveolar Type II Epithelial Cell Line RLE-6TN
- Transport of Prostaglandin E_1 across Rat Erythrocyte Membrane(Biopharmacy)
- プロスタグランジンE_1の腎刷子縁膜輸送系は膜電位感受性であるが, パラアミノ馬尿酸輸送系とは異なる可能性がある
- Effect of Neonatal Exposure of 17β-Estradiol and Tamoxifen on Hepatic CYP3A Activity at Developmental Periods in Rats
- Nanoparticulation of Poorly Water Soluble Drugs Using a Wet-Mill Process and Physicochemical Properties of the Nanopowders
- Mechanism of Insulin Uptake in Rat Alveolar Type II and Type I-Like Epithelial Cells
- EFFECT OF CYP2C POLYMORPHISMS ON THE PHARMACOKINETICS OF PHENYTOIN IN JAPANESE PATIENTS WITH EPILEPSY
- Population Pharmacokinetics of Phenytoin in Japanese Patients with Epilepsy : Analysis with a Dose-Dependent Clearance Model
- TRANSPORT MECHANISMS OF BESTATIN IN INTESTINAL AND RENAL BRUSH-BORDER MEMBRANES
- TRANSPORT MECHANISMS OF β-LACTAM ANTIBIOTICS IN INTESTINAL BRUSH-BORDER MEMBRANES
- TRANSPORT MECHANISMS OF CATIONIC DRUGS IN RAT RENAL BRUSH-BORDER AND BASOLATERAL MEMBRANES
- TRANSPORT OF DRUGS BY PLASMA MEMBRANE VESICLES ISOLATED FROM RAT KIDNEY CORTEX
- TRANSPORT CHARACTERISTICS AND CYTOTOXICITY OF CYCLOSPORIN A IN CULTURED KIDNEY EPITHELIAL CELL LINE (LLC-PK_1)
- Oligonucleotides against a splicing enhancer sequence led to dystrophin production in muscle cells from a Duchenne muscular dystrophy patient
- Fulminant Massive Gas Gangrene Caused by Clostridium perfringens
- PJ-030 Assessment of Myocardial Strain in Duchenne Muscular Dystrophy Patients with Left Ventricular Asynchrohy(Echo/Doppler 3 (I) : PJ5)(Poster Session (Japanese))
- A Female Carrier of Duchenne Muscular Dystrophy Complicated with Cardiomyopathy
- Effects of Exercise Training on Physical Activity in Older People : a Randomized Controlled Trial
- Effect of Thai Plant Extracts on P-glycoprotein Function and Viability in Paclitaxel-Resistant HepG2 Cells
- Beneficial effects of beta-blockers and angiotensin-converting enzyme inhibitors in Duchenne muscular dystrophy
- Correlation between the M and F wave characteristics and the innervated muscle strength in spinal muscular atrophy
- Quantitative Estimation of Dystrophin Protein : A Sensitive and Convenient "Two-Antibody Sandwich" ELISA
- Transport Characteristics of Ribavirin in Human Erythrocyte Membrane Vesicles
- Human Erythrocyte Nucleoside Transporter ENT1 Functions at Ice-cold Temperatures
- Characterization of Protamine Uptake by Opossum Kidney Epithelial Cells