Nitric Oxide and Carbon Monoxide Act as Inhibitory Neurotransmitters in the Longitudinal Muscle of C57BL/6J Mouse Distal Colon
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概要
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The present study was designed to identify the inhibitory neurotransmitters mediating nonadrenergic noncholinergic relaxation in the longitudinal muscle of C57/BL mouse distal colon. Relaxation induced by electrical field stimulation (EFS) was recorded isotonically in the presence of atropine and guanethidine. Cyclic guanosine-3′,5′-monophosphate (cyclic GMP) content was measured by radioimmunoassay. EFS-induced relaxation was inhibited by nitro-L-arginine (L-NNA) and Sn (IV) protoporphyrin dichloride IX (SnPP-IX), a nitric oxide (NO) and carbon monoxide (CO) synthase inhibitor, respectively. A combination of both inhibitors produced an additive effect. ODQ, a soluble guanylate cyclase inhibitor, inhibited EFS-induced relaxation. NOR-1, a NO donor, and carbon monoxide-releasing molecule-2 (CORM-2) treatment relaxed the distal colon and increased cyclic GMP content. The effects of NOR-1 and CORM-2, a CO donor, were inhibited by ODQ. KT5823, a cyclic GMP–dependent protein kinase inhibitor, inhibited EFS-induced relaxation. EFS-induced relaxation in the presence of KT5823 was further inhibited by L-NNA, but not by SnPP-IX. In addition, KT5823 inhibited CORM-2–induced relaxation, but not NOR-1–induced relaxation. H89, a cyclic AMP–dependent protein kinase inhibitor, inhibited EFS-induced relaxation, and EFS-induced relaxation in the presence of H89 was further inhibited by L-NNA. These results suggested that NO and CO function as inhibitory neurotransmitters in the longitudinal muscle of C57BL mouse distal colon.
著者
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Nakajima Hidemitsu
Laboratory of Veterinary Pharmacology, Division of Veterinary Science, Osaka Prefecture University G
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Takeuchi Tadayoshi
Laboratory of Veterinary Pharmacology, Division of Veterinary Science, Osaka Prefecture University G
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Hidaka Ayako
Laboratory Of Dairy Food Science College Of Bioresource Sciences Nihon University
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Azuma Yasu-taka
Laboratory Of Veterinary Pharmacology Division Of Veterinary Science Osaka Prefecture University Gra
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Hidaka Ayako
Laboratory Of Veterinary Pharmacology Graduate School Of Life And Environmental Science Osaka Prefec
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Takeuchi Tadayoshi
Laboratory Of Veterinary Pharmacology Division Of Veterinary Science Osaka Prefecture University Gra
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Nakajima Hidemitsu
Laboratory Of Veterinary Pharmacology Division Of Veterinary Science Osaka Prefecture University Gra
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