Speciation Analysis of Arsenics in Commercial Hijiki by High Performance Liquid Chromatography-tandem-mass Spectrometry and High Performance Liquid Chromatography-inductively Coupled Plasma Mass Spectrometry
スポンサーリンク
概要
- 論文の詳細を見る
Edible brown alga (hijiki in Japanese, Hijikia fusiforme) contains not only a high content of inorganic arsenic (iAs) but also various arsenosugars (AsSugs) which are metabolized to dimethylarsinic acid (DMA) in mammals. Since DMA is considered to be carcinogenic in rodents, it is necessary to accurately measure the contents of AsSugs as well as iAs for the risk assessment of seaweed consumption. Seven commercially available dried-hijiki products and two raw hijiki products were analyzed. Total-As was measured by inductively coupled plasma mass spectrometry (ICP-MS) with the Dynamic Reaction Cell (DRC) mode after acid-digestion. After water-extraction, AsSugs were detected by HPLC-MS/MS with multiple reaction monitoring in the positive ion mode and speciation analysis of arsenics was performed by HPLC-ICP-MS. The ranges of total-As obtained by acid digestion (A-TAs) in nine hijiki samples were 37.1-118.6 μg As/g dry weight (dw), and those of water extracted total-As (W-TAs) were 18.4-81.0 μg As/g dw. The ratios of water extracted iAs (W-iAs) to A-TAs ranged from 24.5 to 60.1%. The major compound detected was arsenate in all samples (8.9-70.5 μg As/g dw). Dimethylarsenosugar sulfate, AsSug 408, showed the highest peak among AsSugs detected. The content ratio of water extracted AsSugs (W-AsS) to A-TAs was estimated to be from 3.7 to 27.6%. The contents of A-TAs, W-iAs and W-AsS varied depending on the hijiki product. HPLC-MS/MS detected AsSugs more sensitively than HPLC-ICP-MS. Since iAs could not be detected by HPLC-MS/MS, combined analysis consisting of HPLC-MS/MS and HPLC-ICP-MS is necessary for accurate determination of arsenic species in seaweed products and also for the toxicological evaluation of AsSugs.
著者
-
Shimoda Yasuyo
Environmental Toxicology and Carcinogenesis Research Unit, Nihon University College of Pharmacy
-
Suzuki Yoshihiro
Research Center for Occupational Poisoning, Tokyo Rosai Hospital
-
Endo Yoko
Research Center for Occupational Poisoning, Tokyo Rosai Hospital
-
Kato Koichi
Environmental Toxicology and Carcinogenesis Research Unit, Nihon University College of Pharmacy
-
Tachikawa Mariko
Environmental Toxicology and Carcinogenesis Research Unit, Nihon University College of Pharmacy
-
Endo Ginji
Department of Preventive Medicine and Environmental Health, Osaka City University Medical School
-
Yamanaka Kenzo
Environmental Toxicology and Carcinogenesis Research Unit, Nihon University College of Pharmacy
-
Endo Yoko
Res. Center For Occupational Poisoning Tokyo Rosai Hospital Japan Labour Health And Welfare Organiza
-
Endo Yoko
Clinical Research Center For Occupational Poisoning Tokyo Rosai Hospital Japan Labour Health And Wel
-
Endo Ginji
Department Of Environmental Health Medical School Osaka City University
-
Yamanaka Kenzo
Environmental Toxicology And Carcinogenesis Res. Unit Nihon Univ. School Of Pharmacy
-
Shimoda Yasuyo
Environmental Toxicology And Carcinogenesis Res. Unit Nihon Univ. School Of Pharmacy
-
Endo Yoko
Research Center For Occupational Poisoning Kansai Rosai Hospital Japan Labour Health And Welfare Org
-
Endo Yoko
Research Center For Occupational Poisoning Tokyo Rosai Hospital Japan Labour Health Organization
関連論文
- Relationship between selected indoor volatile organic compounds, so-called microbial VOC, and the prevalence of mucous membrane symptoms in single family homes
- Speciation Analysis of Arsenics in Commercial Hijiki by High Performance Liquid Chromatography-tandem-mass Spectrometry and High Performance Liquid Chromatography-inductively Coupled Plasma Mass Spectrometry
- Rapid and Effective Speciation Analysis of Arsenic Compounds in Human Urine using Anion-Exchange Columns in HPLC-ICP-MS
- HPLC-ICP-MS Speciation Analysis of Arsenic in Urine of Japanese Subjects without Occupational Exposure
- Sampling Methods and Residential Factors Affecting Formaldehyde Concentration in Indoor Air
- Skin Sensitization Study by Quantitative Structure-Activity Relationships (QSAR)
- A Case of Acute Organotin Poisoning
- EPR Study on Cr^ Distribution in LEC GaAs: Cr Wafers
- Determination of Sub-ppb Cadmium in Urine by Solid-Phase Extraction and Inductively Coupled Plasma-Mass Spectrometry
- Optimization of the Determination of Ortho-phthalaldehyde in Air by Derivatization with 2,4-dinitorophenylhyrazine (DNPH)
- Direct Analysis of Permethrins in Human Blood by SPE-GC/MS
- Simple and Rapid Determination of GTPase Activity by Capillary Electrophoresis without Radioisotope
- SPE-GC/FTD determination of N-methyl-2-pyrrolidone and its metabolites in urine
- Influence of coffee intake on urinary hippuric acid concentration
- Effects of Glutaraldehyde Exposure on Human Health
- Acute Arsine Poisoning Confirmed by Speciation Analysis of Arsenic Compounds in the Plasma and Urine by HPLC-ICP-MS
- 403 EFFECTS OF METALLOTHIONEIN ON THE MUTAGENICITY OF QUERCETIN
- Stability in Urine of Authentic Phenylglyoxylic and Mandelic Acids as Urinary Markers of Occupational Exposure to Styrene
- Low-molecular Weight Amines Have Marked Potential to Promote Colitis in a Mouse Experimental Model: A Possible Proposal of in vivo Formation of Their Chloramines
- Two- and 13-week Inhalation Toxicities of Indium-tin Oxide and Indium Oxide in Rats
- Inhalation Carcinogenicity and Chronic Toxicity of Indium-tin Oxide in Rats and Mice
- Pulmonary Toxicity in Mice by 2- and 13-week Inhalation Exposures to Indium-tin Oxide and Indium Oxide Aerosols
- Direct determination of N-methyl-2-pyrrolidone metabolites in urine by HPLC-electrospray ionization-MS/MS using deuterium-labeled compounds as internal standard
- Development of an analytical method to confirm toxic trimethylated tin in human urine
- Four Cases of Chemical Burns Thought to Be Caused by Exposure to Chromic Acid Mist
- O-10 Study of Arsenicosis in Bangladesh : Relationship of As level in urine and hair and health condition(CARCINOGENICITY)(GENERAL SESSION BY ORAL PRESENTATION)(Proceedings of the 31st Annual Meeting)
- S4-2 Urinary Sulfur-Containing Metabolite Produced by Intestinal Bacteria following Oral Administration of Dimethylarsinic Acid to Rats(SYMPOSIUM 4: ARSENIC IN THE ENVIRONMENT AND THE TOXICITY)(Proceedings of the 31st Annual Meeting)
- Determination of trivalent methylated arsenicals in rat urine by liquid chromatography-inductively coupled plasma mass spectrometry after solvent extraction
- Hemoglobin Adducts as a Marker of Exposure to Chemical Substances, Especially PRTR Class I Designated Chemical Substances
- Fractional Clearances of Low Molecular Weight Proteins in Lead Workers
- A Case of Occupational Bronchial Asthma and Contact Dermatitis Caused by ortho-Phthalaldehyde Exposure in a Medical Worker(Rapid Communication)
- The relationship between exposure to microbial volatile organic compound and allergy prevalence in single-family homes.
- Arsenic speciation analysis of urine samples from individuals living in an arsenic-contaminated area in Bangladesh
- A Guillain-Barre Syndrome-like Neuropathy Associated with Arsenic Exposure
- Development of an Analytical Method for the Determination of Arsenic in Urine by Gas Chromatography-mass Spectrometry for Biological Monitoring of Exposure to Inorganic Arsenic
- Blood pressure components and risk for chronic kidney disease in middle-aged Japanese men : The Kansai Healthcare Study
- Visceral adiposity, not abdominal subcutaneous fat area, is associated with high blood pressure in Japanese men : the Ohtori study
- Development of a Simple and Precise Method to Determine Low Urinary Cadmium Levels by Using Inductively Coupled Plasma-Mass Spectrometry