Mechanism of Action of Tetrandrine, a Natural Inhibitor of Candida albicans Drug Efflux Pumps

スポンサーリンク

概要

• 論文の詳細を見る

•   Synergistic effects have previously been observed for a natural compound, tetrandrine (TET), with fluconazole (FLC) in vitro and in the treatment of Candida albicans-infected mice. To investigate the mechanisms of these synergistic effects, 16 strains of C. albicans from the same parent but with different FLC sensitivities were examined using flow cytometry and fluorescent spectrophotometry. Rhodamine 123 (Rh123)-positive cells and intracellular Rh123 fluorescence intensity were determined in accumulation/efflux experiments involving no or a noncytotoxic dose of TET. Total RNA extracted from each strain was used to compare the expressions of drug efflux pump genes in FLC-susceptible, -susceptible dose-dependent, and -resistant strains before and 24 h after TET administration. Accumulation experiments determined that mean percentages of Rh123-positive cells were 26.65% (TET-free) and 70.99% (TET 30 μg/ml), and mean respective intracellular Rh123 fluorescence intensities were 11.34 and 18.00. Efflux experiments showed that percentages of Rh123-positive cells were 1.79% (TET free) and 42.57% (TET 30 μg/ml), respectively, and respective mean intracellular Rh123 fluorescence intensities were 0.74 and 2.19. Differences in MDR1, FLU1, CDR1, and CDR2 expression levels in the absence of TET were statistically significant (p<0.05) between FLC-susceptible, -susceptible dose-dependent, and -resistant strains. Compared with TET-free conditions, 24 h TET-treated strains showed statistically different (p<0.05) expression of MDR1 (FLC-resistant strain), FLU1 (FLC-susceptible dose-dependent and -resistant strains), and CDR1 and CDR2 (FLC-susceptible, -susceptible dose-dependent, and -resistant strains). Thus TET can inhibit the C. albicans drug efflux system and reduce drug efflux. Its mechanism of action is related to the inhibition of expression of the drug efflux pump genes MDR1, FLU1, CDR1, and CDR2.

著者

• Zhang Hong

  Department Of Biological Sciences Texas Tech University

• GAO Aili

  Department of Dermatology, The First Affiliated Hospital, Jinan University

• LI Fengxia

  Department of Dermatology, The First Affiliated Hospital, Guangdong College of Pharmacy

• ZHANG Gehua

  The Third Affiliated Hospital, Sun Yat-sen University

• HO Hon

  Department of Dermatology, The First Affiliated Hospital, Jinan University

• LIAO Wanqing

  Department of Dermatology, Shanghai Changzheng Hospital, National Laboratory of Medical Mycology, Se

• Ho Hon

  Department Of Dermatology First Affiliated Hospital Jinan University

• Liao Wanqing

  Department Of Dermatology Changzheng Hospital Second Military Medical University

• ZHANG Hong

  Department of Dermatology, The First Affiliated Hospital, Jinan University

• LIAO Wanqing

  Department of Dermatology, Shanghai Changzheng Hospital, National Laboratory of Medical Mycology, Second Military Medical University

関連論文

• EFFECT OF NICARDIPINE, A CALCIUM ANTAGONIST, ON INDUCTION OF PEROXISOMAL ENZYMES BY DEHYDROEPIANDROSTERONE SULFATE IN CULTURED RAT HEPATOCYTES
• A basic study on lesion detectability for hot spot imaging of positron emitters with dedicated PET and positron coincidence gamma camera
• Fundamental study of hot detectability in 3-dimensional positron emission tomography
• Hexokinase-II expression in untreated oral squamous cell carcinoma : comparison with FDG PET imaging
• Adenovirus-mediated p53 gene transfer sensitizes hepatocellular carcinoma cells to heavy-ion radiation
• Germ Cell Loss Induced by ^C^ Ion Irradiation in Young Female Mice
• Algogenic effects of Extract of the Tumor Mass Isolated from Mice with Orthotopic Melanoma Inoculation
• Mechanism of Action of Tetrandrine, a Natural Inhibitor of Candida albicans Drug Efflux Pumps
• A high performance low power 12-bit 40MS/s pipelined ADC
• Response of osteogenic sarcoma to neoadjuvant therapy : evaluated by 18F-FDG-PET
• Soil Erosion Study on Hillside in Southern Jiangsu Province Using the Cesium-137 Tracer Technique (Soil Physics)
• Overexpression of an Arabidopsis Peroxisomal Ascorbate Peroxidase Gene in Tobacco Increases Protection Against Oxidative Stress
• Performance stability of SHR-2000 high resolution PET for animal research
• Clinical Study of High Intensity Focused Ultrasound Treatment for Uterine Fibroids
• Clinical Study of High Intensity Focused Ultrasound Treatment for Uterine Fibroids
• A Low-Power Second-Order Two-Channel Time-Interleaved ΣΔ Modulator for Broadband Applications
• Clinical applications of computerized tomography 3-D reconstruction imaging for diagnosis and surgery in children with large liver tumors or tumors at the hepatic hilum
• Quantitative analysis of propofol in whole blood using capillary electrophoresis
• OE-357 GATA-4 is required for cardiac morphogenesis through transactivation of N-cadherin gene(Development and Differentiation 2 (H) : OE45)(Oral Presentation (English))
• OE-356 Novel Transmembrane Type Class VI Semaphorin, Sema6D Regulates Cardiac Morphogenesis Through PlexinA1(Development and Differentiation 2 (H) : OE45)(Oral Presentation (English))
• The Effect of Hemorrhagic Shock in a Caprine Tibial Fracture Model
• A Time-Variant Analysis of Phase Noise in Series Quadrature Oscillators
• A Time Variant Analysis of Phase Noise in Differential Cross-Coupled LC Oscillators
• Microstructure and Characteristics of SmCoCu/Co Thin Films(Selected papers from MORIS 2006 Workshop on Thermal & Optical Magnetic Materials and Devices)
• Risk factors for sebaceous gland diseases and their relationship to gastrointestinal dysfunction in Han adolescents
• Isolation of Alkaloids from Plant Materials by Combination of Ion-Pair Extraction and Preparative Ion-Pair HPLC Using Sodium Perchlorate. III : Chinese Magnoliae Cortex
• Overexpression of the Arabidopsis 14-3-3 Protein GF14λin Cotton Leads to a "Stay-Green" Phenotype and Improves Stress Tolerance under Moderate Drought Conditions
• The signal transduction mediated by erythropoietin and proinflammatory cytokines in the JAK/STAT pathway in the children with cerebral palsy
• Diazoxide preconditioning alleviates caspase-dependent and caspase-independent apoptosis induced by anoxia-reoxygenation of PC12 cells
• Regulated Expression of an Isopentenyltransferase Gene (IPT) in Peanut Significantly Improves Drought Tolerance and Increases Yield Under Field Conditions
• Characteristic Analysis of Peripheral Blood Mononuclear Cell Apoptosis in Coke Oven Workers
• Formation and Conformation of Baicalin–Berberine and Wogonoside–Berberine Complexes
• Common variants of the UMOD promoter associated with blood pressure in a community-based Chinese cohort

もっと見る 閉じる

スポンサーリンク