Isolation and Characterization of Leptospira spp. from Raccoons in Japan
スポンサーリンク
概要
- 論文の詳細を見る
We investigated whether raccoons (Procyon lotor) carried leptospires in their kidneys in Japan. Leptospira was isolated from 2 of 71 raccoons captured in Kanagawa Prefecture and 1 of 53 raccoons at a zoological park in Nagasaki Prefecture. Anti-Leptospira antibodies were detected in 16 of 124 raccoons (12.9%) in Kanagawa and 33 of 53 raccoons (62.3%) in Nagasaki, respectively. The partial nucleotide sequences of their flaB genes suggested that the isolates belonged to L. interrogans. The serovars of the isolates were identified as Copenhageni/Icterohaemorrhagiae (1 strain in Kanagawa) and Hebdomadis (1 strain both in Kanagawa and Nagasaki) by reactivity with the reference antisera and restriction fragment length polymorphism (RFLP) analysis based on pulsed-field gel electrophoresis and cross-agglutination-absorption test, respectively. RFLP analysis on the serovars Hebdomadis strains revealed genetic diversity among serovar Hebdomadis. Although it is unclear if the raccoons carried leptospires in their kidneys at the time imported, there is no doubt that imported animals are a new reservoir animal of leptospires in Japan.
著者
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KATO Yukio
Department of Dental and Medical Biochemistry, Hiroshima University Graduate School of Biomedical Sc
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UCHIDA Masaki
Department of Biology, National Institute of Polar Research
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Uchida Masaki
Department Of Biology National Institute Of Polar Research
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KUROKI Toshiro
Department of Microbiology, Kanagawa Prefectural Institute of Public Health
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Kuroki Toshiro
Department Of Microbiology Kanagawa Prefectural Institute Of Public Health
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Kato Yukio
Department Of Applied Biological Science Tokyo University Of Science
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KOIZUMI Nobuo
Department of Bacteriology I, National Institute of Infectious Diseases
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MUTO Maki
Department of Bacteriology, National Institute of Infectious Diseases
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MAKINO Takashi
Kanagawa Prefectural Natural Environment Conservation Center
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TAGURI Toshitsugu
Department of Public Health, Nagasaki Prefectural Institute for Environmental Research and Public He
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WATANABE Haruo
Deputy of Director General, National Institute of Infectious Diseases
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TAGURI Toshitsugu
Department of Microbiology, Nagasaki Prefectural Institute for Environmental Research and Public Health
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Koizumi Nobuo
Department of Bacteriology, National Institute of Infectious Diseases
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