Electron Microscopic Study on Lysis of a Cyanobacterium Microcystis

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In a previous study, it was suggested that β-cyclocitral plays an important role in understanding the lysis of cyanobacteria under natural conditions. The present study was conducted in order to understand how the β-cyclocitral lyses cyanobacterial cells, and other anticyanobacterial agents were also investigated. The preliminary study using a scanning electron microscope (SEM) demonstrated that there were three types of morphological changes in the cyanobacterial cells when they were incubated with anticyanobacterial agents: volatile compounds from cyanobacteria cause shrinking and then wrinkling; terpenoids contact directly and cause stripping; basic amino acids cause swelling and then collapsing. In order to clarify the extreme difference in the damage during the morphological changes between the β-cyclocitral and L-lysine(Lys), the transmission electron microscope (TEM) technique was applied. Although a definite difference in the morphological damage was observed, a plausible mechanism for β-cyclocitral could be not deduced. Throughout the experiments using antibiotics, it was found that the apparent morphological changes after lysis did not always correspond to the mode of action.
公益社団法人　日本薬学会の論文