Species-Specific Detection of Listeria monocytogenes Using Polymerase Chain Reaction Assays Targeting the prfA Virulence Gene Cluster
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概要
- 論文の詳細を見る
Sequence comparison of individual genes in the prfA virulence gene cluster (pVGC), a central virulence gene cluster, from different Listeria species indicated that priming sites within the genes appeared to be specific for Listeria monocytogenes exclusively. Therefore, the pVGC was targeted for polymerase chain reaction (PCR) assays to detect and specifically identify L. monocytogenes. Each gene of the pVGC was specifically amplified in L. monocytogenes but not in other Listeria species.
- 社団法人 日本農芸化学会の論文
著者
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KIM Cheol-Ho
Department of Internal Medicine, Seoul National University College of Medicine, Seoul National Unive
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Jung Hye-Jin
Department of Food Science and Technology, College of Industrial Sciences, Chung-Ang University
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Park Sung-Hee
Department of Food Science and Technology, College of Industrial Sciences, Chung-Ang University
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Ha Sang-Do
Department of Food Science and Technology, College of Industrial Sciences, Chung-Ang University
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LEE Kyu-Ho
Department of Environmental Engineering and Biotechnology, Hankuk University of Foreign Studies
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CHUNG Duck
Division of Applied Life Science, Gyeongsang National University
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KIM Min-Gon
Laboratory of Integrative Biotechnology, Korea Research Institute of Bioscience and Biotechnology
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KIM Kwang-Yup
Department of Food Science and Technology, Chungbuk National University
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KIM Keun-Sung
Department of Food Science and Technology, College of Industrial Sciences, Chung-Ang University
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- Species-Specific Detection of Listeria monocytogenes Using Polymerase Chain Reaction Assays Targeting the prfA Virulence Gene Cluster
- Species-Specific Detection of Listeria monocytogenes Using Polymerase Chain Reaction Assays Targeting the prfA Virulence Gene Cluster