酵素膜形状と酵素反応の表面プラズモン共鳴による測定

概要

論文の詳細を見る
An optical biosensor that detects substrates of oxidases was developed as an element of an integrated chemical sensor. The sensor consisted of a spot of layers of enzyme and electron mediator on a thin gold film. The substrate concentration was converted into the refractive index change of electron mediator, and the refractive index was measured by Surface Plasmon Resonance (SPR). SPR was also used to choose the sensing area to give maximum sensitivity within the enzyme spot. SPR detection has advantages in biological binding reaction measurements because it does not need chemical modification for the target molecules, but it had not been suitable for the detection of enzymatic reactions. Our method will enable detection of many types of biological reactions in a uniform SPR measurement format.
社団法人電気学会の論文
2003-04-01