Regulation of Guanylate Cyclase by ATP and Dithiothreitol in Rat Lung Membrane : Involvement of an Insensitive and a Sensitive State to ATP/Dithiothreitol-Stimulation

スポンサーリンク

概要

• 論文の詳細を見る

• ATP/dithiothreitol (DTT)-stimulated guanylate cyclase (GC) in lung membrane was stimulated 18-fold by ATP and DTT, and both its activity and atrial natriuretic peptide (ANP)-stimulated GC activity were observed to be additive. ATP/DTT-stimulated GC was solubilized by octyl glucoside (OG) to examine the mechanism of ATP/DTT-stimulation. GC in OG-extracts was stimulated maximally 2.5-fold by both ATP, ATPγS or AMPPNP, and DTT. preincubation of OG-extracts at 10°C with AMPPNP and DTT (1st-preincubation) converted GC to an insensitive state to stimulation by both ATP and DTT, and this conversion was partly inhibited by a protein phosphatase-1 inhibitor (10 – 1000 nM okadaic acid). On the other hand, ANP-stimulated GC was not converted to an insensitive state to ANP/ATP-stimulation by the 1st-preincubation. Subsequent preincubation of OG-extracts at 10°C with both DTT and, ATP or ATPγS but not AMPPNP converted GC to a state sensitive to ATP/DTT-stimulation, and this conversion was partly inhibited by inhibitors of Ca2+/calmodulin-dependent protein kinase II (KN-62 and KN-93). In contrast, the preincubation with KN-62 and KN-93 had no effect on ANP-stimulated GC activity. The results suggested that phosphorylation was involved in the regulation of ATP/DTT-stimulated GC sensitivity to ATP/DTT-stimulation and that ATP/DTT-stimulated GC activity was likely to be a different type from ANP-stimulated GC activity.

• 社団法人 日本薬理学会の論文
• 2002-05-01

著者

• Asakawa T

  Department Of Pharmacology Faculty Of Medicine Saga Medical School

• Asakawa Takeo

  Department Of Pharmacology Saga Medical School

• Takano Masako

  Department Of Pharmacology Faculty Of Medicine Saga Medical School

• LUO Shiwen

  Department of Pharmacology, Faculty of Medicine, Saga Medical School

• Luo Shiwen

  Department Of Pharmacology Faculty Of Medicine Saga Medical School

• ASAKAWA Takeo

  Department of Pharmacology I, Osaka University School of Medicine

関連論文

• Catecholamine Sensitivity and Cardiac Myosin ATPase Activity in Dogs Treated with Propranolol
• EFFECTS OF PENTOBARBITAL ANESTHESIA ON THE PLASMA CATECHOLAMINES AND RENIN ACTIVITY AS REFLECTED IN THE HEMODYNAMIC CHANGES IN DOGS
• Mechanism and Properties of Inhibition of Purified Rat Brain Adenylate Cyclase by G Protein βγ - Subunits
• F--INDUCED CHANGES AND ITS REVERSAL BY ITP IN MEMBRANE TURBIDITY AND ADENYLATE CYCLASE ACTIVITY OF CHICK BRAIN MICROSOMES
• Regulation of Guanylate Cyclase by ATP and Dithiothreitol in Rat Lung Membrane : Involvement of an Insensitive and a Sensitive State to ATP/Dithiothreitol-Stimulation
• STUDIES ON THE FUNCTIONAL ROLE OF ADENOSINE 3, 5-MONOPHOSPHATE, HISTAMINE AND PROSTAGLANDIN E1 IN THE CENTRAL NERVOUS SYSTEM
• Effects of nipradilol, a new .BETA.-adrenoceptor blocking agent with vasodilating properties, on transmural energy metabolism in the underperfused canine heart.
• G Protein .BETA..GAMMA.-Subunits Inhibit Purified Adenylate Cyclase Independent of the Activation by Ca2+ and Calmodulin.

スポンサーリンク