Cloning and characterization of glucosyltransferase cDNA from Eucalyptus perriniana cultured cells

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概要

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• The Eucalyptus perriniana cultured cells are widely used to biotransform a variety of compounds. The glucosyltransferase activity of a crude protein extract of E. perriniana cultured cells was maximized when cell growth was in the pre-logarithmic to logarithmic phase. We cloned a cDNA encoding glucosyltransferase (EPGT) from E. perriniana cultured cells by RT-PCR using a degenerated primer and RACE-PCR. The cDNA contained an open reading frame encoding 467 amino acids with a calculated molecular mass of 51.6 kDa. The consensus sequence of the plant glucosyltransferases was included in the deduced amino acid sequence. The amino acid sequence of EPGT showed a high identity to glucosyltransferases from tobacco and petunia. The recombinant EPGT was expressed in Escherichia coli and its substrate specificity was examined using UDP-[U-14C] glucose. Cinnamic acid was the best sugar acceptor in the compounds tested.

• 日本植物細胞分子生物学会の論文
• 2004-12-01

著者

• Orihara Yutaka

  Graduate School Of Pharmaceutical Sciences The University Of Tokyo

• YOSHIKAWA Takafumi

  School of Pharmaceutical Sciences, Kitasato University

• NAGASHIMA Shigeyuki

  School of Pharmaceutical Sciences, Kitasato University

• TOMO Satoko

  School of Pharmaceutical Sciences, Kitasato University

• Tomo Satoko

  School Of Pharmaceutical Sciences Kitasato University

• Yoshikawa Takafumi

  School Of Pharmaceutical Sciences Kitasato University

• Nagashima Shigeyuki

  School Of Pharmaceutical Sciences Kitasato University

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• Cloning and characterization of glucosyltransferase cDNA from Eucalyptus perriniana cultured cells
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