Viability of Escherichia coli cells under long-term cultivation in a rich nutrient medium
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概要
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We investigated the viability of Escherichia coli cells during long-term cultivation in Brain Heart Infusion (BHI) medium and observed that the number of viable cells increased, then decreased, and increased again, in this medium, and finally the cells died out within about 10 days. This cell death may result from an increase in the pH of the medium. After repeated cultivation in BHI, bacterial cells that did not die out even under conditions of further cultivation were obtainable from cultures showing a stabilized viable count. We propose that long-term cultivation in BHI medium is a good system for studying growth phase-specific events in E. coli cells, because the total life-cycle of a population of E. coli, including exponential growth, stationary phase, and extinction, can be seen during a period of only about 10 days. Also, this system clearly allows detection of a phenotype that may not be detectable in orther commonly used media. Moreover, in this report, we show that mutants displaying the GASP (growth advantage in stationary phase) phenotype appear at high frequency under long-term cultivation conditions.
- 日本遺伝学会の論文
著者
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Inokuchi H
Department Of Biophysics Graduate School Of Science Kyoto University
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Inokuchi Hachiro
Department Of Biophysics Faculty Of Science Kyoto University
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NAKAHIGASHI Kenji
Department of Biophysics, Graduate School of Science, Kyoto University
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FUKUDA Tomoyuki
Department of Electrical and Electronic Engineering, Faculty of Engineering, Kitami Institute of Tec
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Nakahigashi Kenji
Department Of Biophysics Graduate School Of Science Kyoto University
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Fukuda Tomoyuki
Department Of Biophysics Graduate School Of Science Kyoto University
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