Effective breakage of phage λ DNA by shearing with ceramic-coated needle of syringe
スポンサーリンク
概要
- 論文の詳細を見る
The loss of biological activity of phage λ DNA was much greater when the DNA was sheared using a ceramic-coated needle attached to a syringe compared with a conventional stainless steel needle. Inactivation of the biological activity was due to breakage at the middle of the molecule. The thickness of the ceramic-coating was a crucial factor for the breakage. Because approximately the same level of inactivation was observed with a non-coated needle as with thin glass and quartz tubes, it was concluded that the unknown characteristic(s) of the silicon nitride (SiNx) coating itself resulted in the effective breakage of λ DNA molecules by shearing force.
- 日本遺伝学会の論文
- 2005-02-01
著者
-
Inokuchi Hachiro
Department of Biophysics, Faculty of Science, Kyoto University
-
Inokuti Yukio
Steel Research Laboratory Jfe Steel Co.
-
Inokuchi Hachiro
Department Of Biotechnology National Institute Of Technology And Evaluation
-
Inokuchi Hachiro
Department Of Biophysics Faculty Of Science Kyoto University
関連論文
- Overexpression, Enzymatic Properties and Tissue Localization of a Ferrochelatase of Cucumber
- 大腸菌グルタミンtRNAのアミノ酸特異性変異株-2-変異tRNAのアミノ酸特異性〔英文〕
- 大腸菌グルタミンtRNAのアミノ酸特異性変異株-1-アンチコドン近傍の変異による特異性変化〔英文〕
- Effective breakage of phage λ DNA by shearing with ceramic-coated needle of syringe
- Co-Expression of Yeast Amber Suppressor tRNA^ and Tyrosyl-tRNA Synthetase in Escherichia coli: Possibility to Expand the Genetic Code
- 10Sa RNA Is Associated with 70S Ribosome Particles in Escherichia coli^1
- 10Sa RNA complements the temperature-sensitive phenotype caused by a mutation in the phosphoribosyl pyrophosphate synthetase(prs)gene in Escherichia coli
- Isolation and characterization of a cDNA from soybean and its homolog from Escherichia coli, which both complement the light sensitivity of Escherichia coli hemH mutant strain VS101
- 大腸菌K-12株の新奇なUGAサプレッサ-〔英文〕
- 大腸菌K12株のUGAサプレッサ-活性を示すprfB遺伝子変異の同定〔英文〕
- Control of the availability of exogenous 5-aminolevulinic acid in Escherichia coli
- Viability of Escherichia coli cells under long-term cultivation in a rich nutrient medium
- An E. coli promoter that is sensitive to visible light.
- 10Sa RNA Is Associated with 70S Ribosome Particles in Escherichia coli.