大腸菌と枯草菌で共用できるシャトルベクタ--4-PH300PLKの全核酸塩基配列および形質転換に関する性質〔英文〕

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概要

• 論文の詳細を見る

• The whole nucleotide sequence of pHY300PLK (Ishiwa and Shibahara 1985a) has been determined, pHY300PLK consists of 4870 bp and contains an RNA primer for ori-177 and three open reading frames corresponding to TcR, ApR and Rep-α1. Twenty-three unique restriction endonuclease recognition sites were found. We have confirmed that ORF α1 of pHY300PLK is the plasmid replication gene. A rescue phenomenon has been found with respect to multiple infection by pHY300.2PLK (rep-) and pUB110. A series of experiments indicated that the oligomer form, pHY300.2PLK, was rescued by the monomeric form, pUB110. Additionally, some useful information for using pHY300PLK as a shuttle vector in molecular cloning studies is presented. (Key words: shuttle vector; E, coli; B, subtilis; tetracycline; ampicillin; kanamycin; rescue; MIC).

• 日本遺伝学会の論文

著者

• ISHIWA Hiromi

  Yakult Central Institute for Microbiological Research

• SHIBAHARA-SONE Harue

  Yakult Central Institute for Microbiological Research

• 石和 浩美

  Yakult Institute for Microbiological Research

• 曽根 春恵

  Yakult Central Institute for Microbiological Research

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