大腸菌と枯草菌で共用できるシャトルベクタ--3-pAMα1由来のテトラサイクリン耐性遺伝子とpACYC177由来の複製開始領域のDNAの塩基配列〔英文〕

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概要

• 論文の詳細を見る

• A small plasmid pHY163PLK (2519bp) was constructed from the shuttle vector pHY300PLK. pHY163PLK contains the replication origin of pACYC-177, the tetracycline resistance gene (tetα1) of pAMα1 derived from Streptococcus faecalis and eight continuous unique cloning sites of a polylinker region (EcoRI, SmaI, BamHI, SalI, PstI, BglII, XbaI and HindIII). pHY163PLK, a copy-number mutant, has one base substitution in RNA I region compared with that of p15A which is the ancestor of ori-pHY163PLK. The tetα1, which codes 458 amino acids (1374bp), is highly homologous to the TcR genes of other Gram-positive bacteria but completely different from the TcR gene encoded by pBR322 in Gram-negative bacteria. The regulatory region of tetα1 (constitutive type) has an insertion of seven base pairs in the leader peptide region of a related inducible TcR gene. (key words: tetracycline resistance gene; pAMα1; Streptococcus; nucleotide sequence; amino acid sequence)

• 日本遺伝学会の論文

著者

• ISHIWA Hiromi

  Yakult Central Institute for Microbiological Research

• SHIBAHARA Harue

  Yakult Institute for Microbiological Research

• ISHIWA Hiromi

  Yakult Institute for Microbiological Research

関連論文

• 大腸菌と枯草菌で共用できるシャトルベクタ--4-PH300PLKの全核酸塩基配列および形質転換に関する性質〔英文〕
• 大腸菌と枯草菌で共用できる新しいシャトルベクタ--2-ポリリンカ-を挿入した多目的クロ-ニングベクタ-pHY300PLK〔英文〕
• 大腸菌と枯草菌で共用できるシャトルベクタ--3-pAMα1由来のテトラサイクリン耐性遺伝子とpACYC177由来の複製開始領域のDNAの塩基配列〔英文〕

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