Genetic complementation in hybrid cells derived from mutagen-induced 6-thioguanine-resistant Chinese hamster cells.

スポンサーリンク

概要

• 論文の詳細を見る

• 6-Thioguanine(6TG)-resistant clones were obtained in Chinese hamster cells after treatment with ethyl methanesulfonate (EMS) or N-methyl-N'-nitro-N nitrosoguanidine (MNNG). These 6TG-resistant clones were tested for their complementation ability using cell hybridization method with polyethylene glycol. Among twenty-seven EMS-induced 6TG-resistant clones, six were shown to be complementable with a standard CH 252 clones (group A) and classified as complementation group B. Among fifteen MNNG-induced 6TG-resistant clones, three were classified as group A and ten as group B. Further hybridization tests within the same complementation group showed that there were no subgroups in each complementation group. These results indicate that the chemicals used for the induction of forward mutations may have affected different cistrons in the hypoxanthine-guanine phosphoribosyltransferase locus in cultured Chinese hamster cells.

• 日本遺伝学会の論文

著者

• IWAHARA Shigeo

  Laboratory of Genetics, Hatano Research Institute

• SHIBUYA TOHRU

  Laboratory of Animal Genetics, Faculty of Agriculture Nagoya University

• SAKAI YOSHIKO

  Laboratory of Genetics, Hatano Research Institute, Food and Drug Safety Center

関連論文

• X線照射による雄マウス減数分裂後の染色体異常生成〔英文〕
• Isopropyl methanesulfonateの雄マウス投与による1細胞期胚染色体異常と優性致死誘発との関連性〔英文〕
• MUTAGENICITY TESTING OF ST-FILM WITH THE AMES TEST, CHROMOSOME TEST IN VITRO AND MICRONUCLEUS TEST IN FEMALE MICE
• 日本鶏におけるCp遺伝子の作用〔英文〕
• Genetic complementation in hybrid cells derived from mutagen-induced 6-thioguanine-resistant Chinese hamster cells.
• Studies on chemical induction of chromosomal aberrations in postcopulation germ cells and zygotes of female mice. II. Induction of heritable translocations.:II. Induction of heritable translocations

スポンサーリンク