GroEL Chaperone Binding to Beetle Luciferases and the Implications for Refolding When Co-expressed

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概要

• 論文の詳細を見る

• The folding of many proteins including luciferase in vivo requires the assistance of molecular chaperone proteins. To understand how a chaperone targets luciferase, we took three luciferases that give different bioluminescence with the same luciferin substrate and with differences in homology. The three luciferase genes, firefly luciferase (FF-Luc) (from Pyrocoelia miyako), and red (RE-Luc) and green (GR-Luc) bioluminescence-emitting luciferases (from Phrixothrix railroad-worms), were expressed in Escherichia coli to produce fusion proteins with predicted molecular masses. Subsequently, we observed that DnaK and GroEL were co-purified along with recombinant luciferase. Although the amount of co-purified DnaK was almost the same compared to FF-Luc, GroEL was 25 and 32 times higher in GR-Luc and RE-Luc respectively. Furthermore, co-expression of GroEL/GroES along with luciferase substantially refolded RE-Luc and GR-Luc compared to FF-Luc.

• 社団法人 日本農芸化学会の論文
• 2004-10-23

著者

• OHMIYA Yoshihiro

  Cell Dynamics Research Group, Research Institute for Cell Engineering, National Institute of Advance

• Taguchi Takahisa

  Cell Dynamics Research Group National Institute Of Advanced Industrial Science And Technology

• MORI HIROTADA

  Research and Education Center for Genetic Information,Nara Institute of Science and Technology

• Venkatesh Balan

  Cell Dynamics Research Group National Institute Of Advanced Industrial Science And Technology

• Mori Hirotada

  Research And Education Center For Genetic Information Nara Institute Of Science And Technology

• ARIFUZZAMAN Mohammad

  Research and Education Center for Genetic Information, Nara Institute of Science and Technology

• Arifuzzaman Mohammad

  Research And Education Center For Genetic Information Nara Institute Of Science And Technology

• Ohmiya Yoshihiro

  Cell Dynamics Research Group National Institute Of Advanced Industrial Science And Technology

関連論文

• Improved Expression of Novel Red- and Green-emitting Luciferases of Phrixothrix Railroad Worms in Mammalian Cells
• 3P-227 神経回路網情報処理を司る機能分子の解析(神経回路・脳の情報処理,第46回日本生物物理学会年会)
• Analysis of Organic Solvent Tolerance in Escherichia coli Using Gene Expression Profiles from DNA Microarrays
• GroEL Chaperone Binding to Beetle Luciferases and the Implications for Refolding When Co-expressed
• cDNA Cloning and Characterization of a Secreted Luciferase from the Luminous Japanese Ostracod, Cypridina noctiluca
• Identification of human calphoglin-induced phosphoglucomutase phosphorylation in Escherichia coli

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