cDNA Cloning and Characterization of a Secreted Luciferase from the Luminous Japanese Ostracod, Cypridina noctiluca
スポンサーリンク
概要
- 論文の詳細を見る
A secreted luciferase from the marine ostracod, Vargula hilgendorfii, is a useful tool for gene expression assays in living mammalian cells. We have cloned the cDNA of a new secreted luciferase from the ostracod Cypridina noctiluca, which inhabits the coast of Japan. C. noctiluca luciferase consists of 553 amino acid residues with a molecular mass of 61,415 Da, as deduced from the nucleotide sequence. The homologies of nucleotide and amino acid sequences with V. hilgendorfii luciferase are 79.2% and 83.1%, respectively. C. noctiluca luciferase can expressed in and secreted from cultured mammalian cells. The characteristic properties of expressed C. noctiluca luciferase are similar to those of V. hilgendorfii luciferase. However, the activity of C. noctiluca luciferase in culture medium is much higher than that of V. hilgendorfii luciferase, suggesting that C. noctiluca luciferase is a highly potent reporter enzyme for real-time and continuous monitoring of gene expression in living cells.
- 社団法人 日本農芸化学会の論文
- 2004-03-23
著者
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NAKAJIMA Yoshihiro
Cell Dynamics Research Group, Research Institute for Cell Engineering, National Institute of Advance
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OHMIYA Yoshihiro
Cell Dynamics Research Group, Research Institute for Cell Engineering, National Institute of Advance
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Nakajima Y
Cell Dynamics Research Group The Special Division For Human Life Technology National Institute Of Ad
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KOBAYASHI Koji
Cell Dynamics Research Group, The Special Division for Human Life Technology, National Institute of
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YAMAGISHI Kazutoshi
Cell Dynamics Research Group, The Special Division for Human Life Technology, National Institute of
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ENOMOTO Toshiteru
ATTO Co.
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Ohmiya Yoshihiro
Cell Dynamics Research Group National Institute Of Advanced Industrial Science And Technology
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Yamagishi Kazutoshi
Cell Dynamics Research Group The Special Division For Human Life Technology National Institute Of Ad
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Nakajima Yoshihiro
Cell Dynamics Research Group Research Institute For Cell Engineering National Institute Of Advanced
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Kobayashi Koji
Cell Dynamics Research Group The Special Division For Human Life Technology National Institute Of Ad
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Ohmiya Yoshihiro
Cell Dynamics Research Group The Special Division For Human Life Technology National Institute Of Ad
関連論文
- Improved Expression of Novel Red- and Green-emitting Luciferases of Phrixothrix Railroad Worms in Mammalian Cells
- GroEL Chaperone Binding to Beetle Luciferases and the Implications for Refolding When Co-expressed
- cDNA Cloning and Characterization of a Secreted Luciferase from the Luminous Japanese Ostracod, Cypridina noctiluca