The Positive Effect of the Decreased NADPH-Preferring Activity of Xylose Reductase from Pichia stipitis on Ethanol Production Using Xylose-Fermenting Recombinant Saccharomyces cerevisiae
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概要
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We focused on the effects of a mutation of xylose reductase from Pichia stipitis (PsXR) on xylose-to-ethanol fermentation using recombinant Saccharomyces cerevisiae transformed with PsXR and PsXDH (xylitol dehydrogenase from P. stipitis) genes. Based on inherent NADH-preferring XR and several site-directed mutagenetic studies using other aldo-keto reductase enzymes, we designed several single PsXR mutants. K270R showing decreased NADPH-preferring activity without a change in NADH-preferring activity was found to be a potent mutant. Strain Y-K270R transformed with K270R PsXR and wild-type PsXDH showed a 31% decrease in unfavorable xylitol excretion with 5.1% increased ethanol production as compared to the control in the fermentation of 15 g l−1 xylose and 5 g l−1 glucose.
- 社団法人 日本農芸化学会の論文
著者
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Makino Keisuke
Institute of Advanced Energy, Kyoto University
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PACK Seung
Institute of Advanced Energy, Kyoto University
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KODAKI Tsutomu
Institute of Advanced Energy, Kyoto University
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Watanabe Seiya
Institute Of Advanced Energy Kyoto University:crest Japan Science And Technology Agency(jst):new Ene
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WATANABE Seiya
Faculty of Engineering, Kyoto University
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SALEH Ahmed
Institute of Advanced Energy, Kyoto University
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ANNALURU Narayana
Institute of Advanced Energy, Kyoto University
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