In Vitro Synthesis of a Larger Precursor of Tobacco β-1, 3-Glucanase
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概要
- 論文の詳細を見る
Translation of total RNAprepared from cultured tobacco cells by the guanidine-HCl method in a cell-free reticulocyte lysate system results in the synthesis of a larger precursor of β-1, 3- glucanase (EC 3.2.1.39). This identification was based on immunoprecipitation with specific anti-β- 1, 3-glucanase antibody. Furthermore, sodium dodecyl sulfate polyacrylamide gel electrophoresis after limited proteolysis and two dimensional tryptic fingerprintings of β-1, 3-glucanase and its precursor show a high degree of similarity. The precursor is larger than the mature protein by 4, 000 daltons. β-1, 3-Glucanase mRNAwas partially purified by oligo(dT)-cellulose column chromatography and sucrose density gradient centrifugation. The sedimentation coefficient of the mRNAwas estimated to be about 17S.
- 社団法人 日本農芸化学会の論文
著者
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Kato Kunio
Central Research Institute The Japan Tobacco & Salt Public Corporation
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Kato Kunio
Central Research Institute, Japan Tobacco and Salt Public Corporation
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Shinshi Hideaki
Central Research Institute, Japan Tobacco and Salt Public Corporation
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