Physical and Chemical Properties of β-3-1, 3-Glucanase from Cultured Tobacco Cells
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概要
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β-1, 3-Glucanase (EC 3.2.1.39) is one of the major soluble proteins of cultured tobacco cells but not of leaf tissues. The enzyme activity in cultured cells increased with culture age, physical and chemical properties of this enzymewere characterized. The enzymecontained about 1 %carbohydrate consisting of only arabinose residue. Its isoelectric point was at pH 9.9.The amino acid composition indicated relatively low content of polar amino acids. The molecular weight of the enzyme estimated by sedimentation equilibrium and sodium dodecyl sulfate gel electrophoresis was 32, 000 and 33, 000, respectively, indicating that the enzyme consists of a single polypeptide chain.
- 社団法人 日本農芸化学会の論文
著者
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Kato Kunio
Central Research Institute The Japan Tobacco & Salt Public Corporation
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Kato Kunio
Central Research Institute, Japan Tobacco and Salt Public Corporation
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Shinshi Hideaki
Central Research Institute, Japan Tobacco and Salt Public Corporation
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