Preparation and Characterization of Phosvitin from Hens Egg Yolk Granule

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Soluble delipidated granule was fractionated into mainly two components by gel filtration and each component was purified by DEAE-cellulose chromatography. The phosvitins so prepared were designated as phosvitin 1 and phosvitin 2. Phosvitin 1 contained 59.2% serine and 10.2% phosphorus, while phosvitin 2 had 51.5% serine and 8.9% phosphorus. Phosvitin 1 was dissociated into nine protein fractions ranging in molecular weights from 1.33×104-1.36×105 daltons, in the SDS-polyacrylamide gel electrophoretic pattern. On the other hand, Phosvitin 2 was dissociated into thirteen bands ranging in molecular weights from 1.1×104-1.37×105 daltons, in t e SDSpolyacrylamide gel electrophoretic pattern. Onthe contrary, the major component of phosvitin 1 has a molecular weight of 2.29×104 when determined by gel filtration with 6m guanidine hydrochloride, while the major components ofphosvitin 2 have molecular weights of 2.29×104 and 1.0×104. From these results, it seems that phosvitin is composed of several subcomponents.
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