Synthesis of (1→2)-β-D-Glucan by Cell-free Extracts of Agrobacterium radiobacter IFO 12665b1 and Rhizobium phaseoli AHU 1133

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概要

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• Particulate preparations from Agrobacterium radiobacter IFO 12665b1 and Rhizobium phaseoli AHU 1133 have been shown to catalyze the synthesis of (1→2)-β-D-glucan from UDP-D- [14C]glucose. The (1→2)-β-D-glucans synthesized are suggested to be in a cyclic form without other glycosidic linkages and to consist of a mixture of several components with degrees of polymerization of 17 and more. The enzyme systems from A. radiobacter IFO 12665b1 and R. phaseoli AHU 1133 both required Mn2+ and had optimum activities at pH 7.5-8, and their Km values for UDPD-[14C]glucose were 5×10-5M and 3.3×10-5M, respectively.

• 社団法人 日本農芸化学会の論文

著者

• Amemura Akinori

  Institute Of Scientific And Industrial Research Osaka University

• AMEMURA Akinori

  Institute of Scientific and Industrial Research, Osaka Univeristy

関連論文

• Extracellular Polysaccharides of Rhizobium from the Bangkok MIRCEN Collection
• Isolation of (1→2)-β-D-Glucans from Tropical Strains of Rhizobium and Determination of Their Degrees of Polymerization
• Structural Studies on New, Non-reducing Oligosaccharides Produced by Rhizobium meliloti J7017
• Synthesis of (1→2)-β-D-Glucan by Cell-free Extracts of Agrobacterium radiobacter IFO 12665b1 and Rhizobium phaseoli AHU 1133
• Structural Studies on an Extracellular Acidic Polysaccharide(APS-I) of Rhizobium meliloti 201

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