In Vivo Intracellular Recording Suggests That Gray Matter Astrocytes in Mature Cerebral Cortex and Hippocampus Are Electrophysiologically Homogeneous

スポンサーリンク

概要

• 論文の詳細を見る

• Previous anatomical and in vitro electrophysiology studies suggest that astrocytes are heterogeneous in physiology, morphology, and biochemical content. However, the extent to which this diversity applies to in vivo conditions is largely unknown. To characterize and classify the physiological and morphological properties of cerebral cortical and hippocampal astrocytes in the intact brain, we performed in vivo intracellular recordings from single astrocytes using anesthetized mature rats. Astrocytes were classified based on their glial fibrillary acidic protein (GFAP) immunoreactivity and cell body locations. We analyzed morphometric measures such as the occupied volume and polarity, as well as physiological characteristics such as the mean membrane potential. These measurements did not show obvious segregation into subpopulations, suggesting that gray matter astrocytes in the cortex and hippocampus are composed of a homogeneous population in mature animals. The membrane potential of astrocytes in both cortex and hippocampus fluctuated within a few millivolts in the presence of spontaneous network activity. These membrane potential fluctuations of an astrocyte showed a significant variability that depended on the local field potential state and cell body location. We attribute the variability of the membrane potential fluctuations to local potassium concentration changes due to neuronal activity.

著者

• 熊谷 恒子

  筑波大学生物科学

関連論文

• 昆虫の脳における匂い情報の処理と行動発現
• 白色雑音を用いた神経系の非線形性の解析
• コオロギ気流感覚毛の機械特性の測定
• 昆虫気流感覚細胞の感度向上に寄与する内部雑音の独立性
• In Vivo Intracellular Recording Suggests That Gray Matter Astrocytes in Mature Cerebral Cortex and Hippocampus Are Electrophysiologically Homogeneous
• 昆虫気流感覚毛の機械設計の解析
• Astrocyte Calcium Signaling Transforms Cholinergic Modulation to Cortical Plasticity In Vivo

スポンサーリンク