Role of intestinal Bifidobacterium pseudolongum in dietary fructo-oligosaccharide inhibition of 2,4-dinitrofluorobenzene-induced contact hypersensitivity in mice

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概要

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• Strategies to manipulate the gut microbiota have been explored for preventing allergy development. We previously showed that dietary supplementation with fructo-oligosaccharide (FOS) reduced 2, 4-dinitrofluorobenzene (DNFB)-induced contact hypersensitivity (CHS) in BALB/c mice. Because the CHS response was negatively correlated with the number of faecal bifidobacteria, particularly Bifidobacterium pseudolongum, the present Study aimed to examine whether oral administration of B. pseudolongum affects CHS response. Viable B. pseudolongum was successfully isolated from mouse faeces. Female BALB/c mice were fed a synthetic diet with or without FOS supplementation, and B. pseudolongum (2 X 10^[7] cells) was administered daily throughout the experimental period. Two weeks after starting the test diets, mice received DNFB on the ear auricle twice at 7-d intervals. Conventional cultivation and molecular biological analyses based on 16S rRNA gene sequences showed that administration of FOS and B. pseudolongum resulted in higher excretion of viable bifidobacteria, mainly B. psendolongum. Although dietary FOS reduced the CHS response as demonstrated by ear swelling, B. pseudolongum administration resulted in a reduction in the initial phase only of the CHS response. B. pseudolongum administration increased hapten-specific IgGI, while dietary FOS decreased IgG2a in sera. Administration of FOS and B. pseudolongum decreased interferon-γ production and increased IL-10 production in cervical lymph node cells restimulated with hapten in vitro. We conclude that B. pseudolongum proliferation in the intestinal tract is partially responsible for the reduction in DNFB-induced CHS response by dietary Supplementation with FOS in mice, which may be mediated by the modulation of antigen-induced cytokine production.

• Cambridge University Pressの論文

著者

• FUJIWARA Reiko

  Graduate School of Life Science, Hokkaido University

• SASAJIMA Naho

  Graduate School of Life Science, Hokkaido University

• TAKEMURA Naoki

  Graduate School of Life Science, Hokkaido University

• SONOYAMA Kei

  Research Faculty of Agriculture, Hokkaido University

• Sonoyama Kei

  Research Faculty Of Agriculture Hokkaido University

• Fujiwara Reiko

  Graduate School Of Life Science Hokkaido University

• Sasajima Naho

  Graduate School Of Life Science Hokkaido University

• Takemura Naoki

  Graduate School Of Life Science Hokkaido University

• Watanabe Jun

  Creative Research Initiative Sousei Hokkaido University

関連論文

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