Temperature effect on separation performance in capillary chromatography based on tube radial distribution of aqueous-organic mixture carrier solvents
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概要
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我々は,フューズドシリカ,ポリエチレン,またはポリテトラフルオロエチレン製のオープンキャピラリーと水-親水性-疎水性有機溶媒混合キャリア溶液を用いたキャピラリークロマトグラフィーを開発した.この管径方向分配クロマトグラフィー(TRDC)システムは,層流条件下で実行される.これまでに,フューズドシリカキャピラリーと水-アセトニトリル-酢酸エチル混合キャリア溶液を用いて,TRDCシステムにおける分離挙動の温度の影響について調べた.モデル試料中の1-ナフトールと2,6-ナフタレンジスルホン酸は,有機溶媒過剰キャリア溶液を用いて温度範囲5-23℃でベースライン分離して溶出した.この研究では,キャリア溶液の管径方向分配に基づいたキャピラリーチューブ内でのinner相とouter相の形成が一定であるという仮定のもと,様々なキャピラリー温度で得られたクロマトグラムにvan't Hoffの式を適用することを試みた.We have developed a capillary chromatography system using an open capillary tube made of fused-silica, polyethylene, or poly(tetrafluoroethylene), and a water-hydrophilic/hydrophobic organic mixture carrier solution. This so-called tube radial distribution chromatography (TRDC) system works under laminar flow conditions. Previously, the effects of capillary temperature on separation performance in the TRDC system were examined using a fused-silica capillary tube and a water-acetonitrile-ethyl acetate mixture carrier solution; 1-naphthol and 2,6-naphthalenedisulfonic acid in a model mixture were eluted with baseline separation over the temperature range of 5 – 23°C with the organic solvent-rich carrier solution. In this study, based on the assumption that the phase distribution of inner and outer phases in the capillary tube induced by the tube radial distribution of the carrier solvents did not change with temperature over a limited range, we attempted to apply the van't Hoff equation to the chromatographic data obtained at various capillary temperature.
- 2010-10-31
著者
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Tsukagoshi Kazuhiko
Department of Chemical Engineering and Materials Science, Faculty of Engineering, Doshisha Universit
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Jinno Naoya
Department of Chemical Engineering and Materials Science, Faculty of Science and Engineering, Doshis
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Hashimoto Masahiko
Department of Chemical Engineering and Materials Science, Faculty of Science and Engineering, Doshis
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