若年者精巣上体炎患者におけるクラミジアトラコマチス検出法としての新しいPolymerase chain reaction法の臨床的検討

概要

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15名の若年者精巣上体炎患者から得られた尿及び尿道スワブを対象とした.クラミジアトラコマチスによる尿道炎はEIA法では僅かに20%に認められたのみであったが,PCR法では87%の患者に認められた.この差は統計学的に有意であった.13例のPCR陽性例のうちEIA法でも陽性と判定された症例は僅かに3例のみであった.以上より,PCR法は男の尿中におけるクラミジアトラコマチス検出に感受性が高く,急性精巣上体炎患者におけるクラミジア感染症の非侵襲的なスクリーニング法として有用であるSpecimens from 15 young patients presenting with acute epididymitis were tested for the presence of Chlamydia trachomatis by an enzyme immunoassay (EIA), polymerase chain reaction (PCR), and for other bacteria by standard laboratory techniques. C. trachomatis urethral infection was detected in 3 patients by an EIA test of the urethral swabs (20%) and in 13 patients by the PCR (87%). This difference in detection rate was statistically significant (p < 0.005). Thirteen specimens were positive by the PCR, but only three of them were positive by the EIA method. These findings indicate that the PCR assay is a highly sensitive assay for the detection of C. trachomatis in male urine specimens and provides a noninvasive technique for routine screening of chlamydia infection in the patient with acute epididymitis.