ActinとMyosinの相互作用に関する研究 II. Myosin B, MyosinおよびActinに対する Polyethylene Glycol の作用
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The effect of PEG on myosin B, myosin and actin prepared from rabbit skeletal muscle was studied and the following results were obtained. 1) The KCl concentration-Mg++ ATPase activity curve shifted to right by 30 mM KCｌ. In the presence of PEG, the inhibitory effect of higher KCl concentrations on the Mg++ -ATPase activity of myosin B was less than that observed in the absence of PEG. 2) As seen in the pCa-Mg++ ATPase activity curve of myosin B, Mg++ -ATPase activity was markedly enhanced by PEG at all pCa. In the presence and absence of PEG, maximal activity and half maximal activation of Mg++ -ATPase of myosin B were observed at pCa 5.5 and pCa 6.2-6.4, respectively. Superprecipitation of myosin B was strongly activated by PEG at all pCa, but the extent was smaller at lower pCa. 3) Ca++ -ATPase activity of myosin was activated only about 10% by 10 mg/m? PEG, but it was slightly inhibited by PEG concentrations higher than 10 mg/m? . 4) The turbidity of myosin was increased by an addition of PEG at a lower ionic strength than 0.15 and was not changed at a higher ionic strength exceeding 0.15. On the other hand, the viscosity was increased by PEG even at 0.2 M KCl. 5) Polymerization rate of G-actin was not significantly influenced by PEG. 6) In the presence of Mg++ , ATPase activity of myosin which was pretreated with PEG was only slightly activated by additions of various concentrations of actin. On the other hand, ATPase activity of myosin which was not pretreated with PEG was activated by approximately 60-70% of control by the PEG-pretreated actin in higher concentrations. From these results, it is indicated that PEG does not affect the troponin-tropomyosin complex and actin, but has a marked influence on myosin.
- 1975-04-30
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