γ-Polyglutamic acid-coated vectors for effective and safe gene therapy.
スポンサーリンク
概要
- 論文の詳細を見る
In the present study, we developed some novel gene delivery vectors, coated cationic complexes with gamma-polyglutamic acid (gamma-PGA) for effective and safe gene therapy. Cationic complexes were constructed with pDNA and cationic vectors, such as poly-L-arginine hydrochloride (PLA), poly-L-lysine hydrobromide (PLL), N-[1-(2, 3-dioleyloxy) propyl]-N, N, N-trimethylammonium chloride (DOTMA)-cholesterol (Chol) liposomes, and DOTMA-dioleylphosphatidylethanolamine (DOPE) liposomes. The cationic complexes showed high gene expression with strong cytotoxicity in melanoma B16-F10 cells. The cationic complexes were also strongly toxic to erythrocytes. On the other hand, the gamma-PGA was able to coat all cationic complexes and form stable nano-sized particles with negative charges. These gamma-PGA-coated complexes had high gene expression without cytotoxicity and toxicities to the erythrocytes. In in vivo transfection experiments, polyplexes showed high transfection efficiency over 10(5) RLU/g in the lung tissue after intravenous injection, although gamma-PGA-coated polyplexes showed a high value in the spleen. High transfection efficiency in lipoplexes and gamma-PGA-coated lipoplexes was observed in the spleen and lung. Thus, gamma-PGA-coated vectors are useful for clinical gene therapy.
- 2010-03-19
論文 | ランダム
- Targeting chemotherapy for malignant brain tumor using thermosensitiveliposome and localized hyperthermia
- 当院における僧帽弁疾患に対するmaze手術の初期成績--洞調律維持の予測因子と心房収縮の経時的変化--
- Drug delivery to the brain using thermosensitive liposome and localhyperthermia
- 悪性脳腫瘍に対する熱感受性リポソーム脳組織内加温による化学温熱療法
- 疣贅様エコーを有する患者の臨床背景の解析