Isolation and characterization of a novel chondroitin sulfate from squid liver integument rich in N-acetylgalactosamine(4,6-disulfate) and glucuronate(3-sulfate) residues

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概要

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• Novel chondroitin sulfate (CS) chains with an average molecular mass of 79.6 kDa were purified from squid liver integument. A compositional analysis of the CS chains using chondroitinases (CSases) ABC and AC-I revealed a range of variably sulfated disaccharides with GlcAβ1-3GalNAc(6-O-sulfate), GlcAβ1-3GalNAc(4-O-sulfate), and GlcAβ1-3GalNAc(4,6-O-disulfate) as the major ones, significant amounts of rare 3-O-sulfated GlcA-containing disaccharides, and a small amount of nonsulfated GlcAβ1-3GalNAc. The CS chains exhibited neurite outgrowth-promoting activity toward embryonic mouse hippocampal neurons, which was abolished completely by digestion with CSase ABC or AC-I. Consequently, whether these CS chains interact with heparin-binding growth factors was tested in a BIAcore system. All of the growth factors exhibited concentration-dependent and specific binding. CS chains from squid liver integument, with their unique composition and strong biological activities, may be a good candidate for therapeutic application.

• Elsevier Ltd.の論文
• 2009-08-17

著者

• Sugahara Kazuyuki

  神戸薬科大学 生化

• Sugahara Kazuyuki

  Hokkaido Univ. Sapporo

• Yamada Shuhei

  Department Of Biochemistry Kobe Pharmaceutical University

• Sugahara Kazuyuki

  Dept. Of Biochemistry. Kobe Pharmaceutical University

• Yamada S

  Department Of Bioscience And Biotechnology Fuculty Of Engineering Okayama University

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