Simple and inexpensive method for cryopreservation of fish sperm combining straw and powdered dry ice.
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概要
- 論文の詳細を見る
Here, we propose a simple and inexpensive method for fish sperm cryopreservation. Sperm samples of the loach Misgurnus anguillicaudatus (Teleostei: Cobitidae) were diluted 7-fold by an extender containing 63.5mM NaCl, 114mM KCl, 20mM Tris and 10% methanol. The cryogenic straws were placed in three kinds of self-made tubes which diameter was changed by commercially available materials and then immersed into powdered dry ice for 2 min and plunged into liquid nitrogen. This procedure resulted in a cooling rate at -421.4±119.84 (control), -55.8 ± 4.32 (tube 1), -40.2 ± 3.43 (tube 2) and -33.3 ± 2.09ºC/min (tube 3). In the slowest cooling rate by the tube 3, total motility (72 ± 3%), duration (146 ± 12s) and hatching rates (29 ± 04%) were higher than those by other rates. Progressive motility (83±5%) did not differ significantly from fresh samples.
- CryoLettersの論文
- 2008-09-01
著者
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ARIAS-RODRIGUEZ Lenin
Graduate School of Fisheries Sciences, Hokkaido University
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Arias-rodriguez Lenin
Graduate School Of Fisheries Sciences Hokkaido University
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Arai K
Laboratory Of Breeding Science Graduate School Of Fisheries Science Hokkaido University
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