The Germ Cell Lineage Identified by vas-mRNA during the Embryogenesis in Goldfish
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概要
- 論文の詳細を見る
vas RNA has been identified in germ-line cells and its precursors in zebrafish, with the result that the germ-line lineage can be traced throughout embryogenesis. In the present study, we described vas localization and the migration of vas-positive cells in goldfish, using whole mount in situ hybridization. The signals of vas mRNA localization appeared at the marginal part of the first to third cleavage planes. The eight signals were detected during the period from the 8- cells to the 512-cell stage. At the late-blastula stage, additional numbers of vas-positive cells were observed, suggesting the proliferation of these cells. At the segmentation period, vas-positive cells showed a long extended distribution along the embryonic axis, but did not form any clusters. vas-positive cells were occasionally distributed at the head region, especially around the future otic vesicle. These signals were inherited to the primordial germ cells, suggesting that vas-positive cells were primordial germ cells (PGCs) in goldfish.
- 社団法人日本動物学会の論文
- 2002-05-25
著者
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YAMAHA Etsuro
Nanae Fresh Water Laboratory, Field Science Center for Northern Biosphere, Hokkaido University
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ARAI Katsutoshi
Laboratory of Breeding Science, Graduate School of Fisheries Science, Hokkaido University
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Otani Satoshi
Laboratory of Breeding Science, Graduate School of Fisheries Sciences, Hokkaido University
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Maegawa Shingo
Molecular and Developmental Biology, Graduate School of Biological Sciences, Nara Institute of Scien
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Inoue Kunio
Molecular and Developmental Biology, Graduate School of Biological Sciences, Nara Institute of Scien
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Yamaha Etsuro
Nanae Fresh Water Laboratory Field Science Center For Northern Biosphere Hokkaido University
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Yamaha Etsuro
Nanae Fish Culture Experimental Station Faculty Of Fisheries Hokkaido University
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Otani Satoshi
Laboratory Of Breeding Science Graduate School Of Fisheries Sciences Hokkaido University
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Inoue K
Molecular And Developmental Biology Graduate School Of Biological Sciences Nara Institute Of Science
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Maegawa Shingo
Molecular And Developmental Biology Graduate School Of Biological Sciences Nara Institute Of Science
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Inoue Kunio
Molecular And Developmental Biology Graduate School Of Biological Sciences Nara Institute Of Science
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Arai K
Laboratory Of Breeding Science Graduate School Of Fisheries Science Hokkaido University
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Arai Katsutoshi
Laboratory Of Breeding Science Graduate School Of Fisheries Sciences Hokkaido University
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Arai Katsutoshi
Grad. Sch. Of Fish. Sci. Hokkaido Univ.
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