Developmental Stages and Germ Cell Lineage of the Loach (Misgurnus anguillicaudatus)

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概要

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• The staging of embryonic and larval development, and the germ cell lineage of the loach, Misgurnusanguillicaudatus, are described. Fertilized eggs were obtained by artificial insemination. For theconvenience of detailed observation and photography of the external appearance, we use dechorionatedembryos. Through a series of operations, these embryos were cultured at 20°C in an incubator.Embryonic and larval development of the loach was divided into five periods: cleavage,blastula, gastrula, segmentation, and hatching. Stages were assigned within each of these periods.Developmental stages were determined and named by morphological features and somite number.The staging series were photographed and tabulated. The germ cell lineage was then elucidatedby whole mount in situ hybridization of mRNA expression of the germ-cell-specific marker vasa andhistological analysis. Primordial germ cells (PGCs) of the loach derived from the cleavage furrowsof 8-cell stage embryos began proliferation in the late blastula period and migrated to the gonadalanlagen through a migration pathway similar to that of the zebrafish. However, it is characteristicof the loach that PGCs migrate a long distance and stay in the posterior part of the yolk-extensionregion.

• 日本動物学会の論文
• 2006-11-25

著者

• SAKAO Suzu

  Laboratory of Breeding Science, Graduate School of Fisheries Science, Hokkaido University

• FUJIMOTO Takafumi

  Laboratory of Breeding Science, Graduate School of Fisheries Science, Hokkaido University

• YAMAHA Etsuro

  Nanae Fresh Water Laboratory, Field Science Center for Northern Biosphere, Hokkaido University

• ARAI Katsutoshi

  Laboratory of Breeding Science, Graduate School of Fisheries Science, Hokkaido University

• Saito Taiju

  Nanae Fresh-Water Laboratory, Field Science Center for Northern Biosphere, Hokkaido University

• kataoka Takashi

  Laboratory of Breeding Science, Graduate School of Fisheries Sciences, Hokkaido University

• Yamaha Etsuro

  Nanae Fresh Water Laboratory Field Science Center For Northern Biosphere Hokkaido University

• Yamaha Etsuro

  Nanae Fish Culture Experimental Station Faculty Of Fisheries Hokkaido University

• Kataoka Takashi

  Laboratory Of Aquaculture Genetics And Genomics Division Of Marine Life Science Graduate School Of F

• Arai K

  Laboratory Of Breeding Science Graduate School Of Fisheries Science Hokkaido University

• Arai Katsutoshi

  Laboratory Of Breeding Science Graduate School Of Fisheries Sciences Hokkaido University

関連論文

• Artificially induced tetraploid masu salmon have the ability to form primordial germ cells
• Restoration of the loach, Misgurnus anguillicaudatus, from cryopreserved diploid sperm and induced androgenesis
• Cryopreservation of Primordial Germ Cells by Rapid Cooling of Whole Zebrafish (Danio rerio) Embryos
• Reproductive capacity of neo-tetraploid loaches produced using diploid spermatozoa from a natural tetraploid male
• Genetic and reproductive potential of spermatozoa of diploid and triploid males obtained from interspecific hybridization of Misgurnus anguillicaudatus female with M. mizolepis male
• Ploidy manipulation using diploid sperm in the loach, Misgurnus anguillicaudatus : a review
• Developmental biotechnology for aquaculture, with special reference to surrogate production in teleost fishes
• Drastic mortality in tetraploid induction results from the elevation of ploidy in masu salmon Oncorhynchus masou
• DIFFERENTIATION OF PRIMORDIAL GERM CELLS FROM EGG IRRADIATED WITH ULTRAVIOLET RAY BEFORE FERTILIZATION IN THE LOACH(Developmental Biology,Abstracts of papers presented at the 76^ Annual Meeting of the Zoological Society of Japan)
• Reproductive capacity of triploid loaches obtained from Hokkaido Island, Japan
• THE DEVELOPMENTAL PROPERTIES OF THE NUCLEO-CYTOPLASMIC HYBRID BETWEEN LOACH AND GOLDFISH(Developmental Biology,Abstracts of papers presented at the 75^ Annual Meeting of the Zoological Society of Japan)
• Embryonic Stages from Cleavage to Gastrula in the Loach Misgunus anguillicaudatus
• Spontaneous mosaicism occurred in normally fertilized and gynogenetically induced progeny of the kokanee salmon Oncorhynchus nerka
• Germ Cell Lineage from a Single Blastomere at 8-Cell Stage in Shiro-uo (ice goby)
• A Cryptic Clonal Line of the Loach Misgurnus anguillicaudatus (Teleostei: Cobitidae) Evidenced by Induced Gynogenesis, Interspecific Hybridization, Microsatellite Genotyping and Multilocus DNA Fingerprinting
• The Germ Cell Lineage Identified by vas-mRNA during the Embryogenesis in Goldfish
• Histological Differentiation of Primordial Germ Cells in Zebrafish
• A sperm cryopreservation protocol for the loach Misgurnus anguillicaudatus and its applicability for other related species
• Inter-populational difference in microsatellite-centromere map distances in the loach, Misgurnus anguillicaudatus
• Simple and inexpensive method for cryopreservation of fish sperm combining straw and powdered dry ice.
• Dorso-ventral axis perturbation in goldfish embryos caused by heat- and pressure-shock treatments for chromosome set manipulation
• AUTONOMOUS DIFFERENTIATION OF ECTOPIC PRIMORDIAL GERM CELLS (PGCS) IN ZEBRAFISH(Developmental Biology)(Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
• Developmental Stages and Germ Cell Lineage of the Loach (Misgurnus anguillicaudatus)
• Disruption of normal meiosis in artificial inter-populational hybrid females of Misgurnus loach
• Sexual dimorphism of gonadal structure and gene expression in germ cell-deficient loach, a teleost fish
• ADULT INDIVIDUALS OBTAINED FROM EMBRYOS RECONSTRUCTED BY NUCLEAR TRANSFER OF THE ADULT CAUDAL FIN CELLS IN MEDAKA(Developmental Biology,Abstracts of papers presented at the 76^ Annual Meeting of the Zoological Society of Japan)
• Meiotic hybridogenesis in triploid Misgurnus loach derived from a clonal lineage
• Visualization and motility of primordial germ cells using green fluorescent protein fused to 3'UTR of common carp nanos-related gene
• Highly efficient induction of triploid Pacific abalone Haliotis discus hannai by caffeine treatment
• Cryopreservation diluents for spermatozoa of Sakhalin taimen Hucho perryi
• The Elimination and Duplication of Lower Part of Blastoderm Effects on the Number of Primordial Germ Cells in Goldfish
• Dorsal specification in blastoderm at the blastula stage in the goldfish, Carassius auratus
• Chimeric Fish Produced by Exchanging Upper Parts of Blastoderms in Goldfish Blastulae
• Cryopreservation of Sakhalin Taimen Hucho perryi spermatozoa: effect of cryoprotectants on post-thaw fertility
• Cold-shock eliminates female nucleus in fertilized eggs to induce androgenesis in the loach (Misgurnus anguillicaudatus), a teleost fish.
• Germ cells are not the primary factor for sexual fate determination in goldfish

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