Plant Regeneration from Hypocotyl Section and Callus in Buckwheat (Fagopyrum esculentum Moench. )

概要

論文の詳細を見る
A tissue culture experiment on buckwheat hypocotyl was carried out by using B5and MS media supplemented with various concentrations of 2,4-D and 6-BA. In thepresence of 1.0-2.0 mg/l 2,4-D, callus induction was frequent and it was enhanced bythe addition of 6-BA. Callus was also induced on the B5 media that contained only6-BA (0.5-2. 0 mg/l). Adventitious shoots were obtained from hypocotyl directly whenthey were cultured on the MS medium with 2.0 mg/l 2,4-D and 2.0 mg/l 6-BA. Thecalli induced in the media with 2.0mg/l 2,4-D and 1.0-2.0 mg/l 6-BA and successivelytransferred to the media without 2,4-D regenerated plantlets, but those induced withlower concentrations of 6-BA did not. This suggests the effect of 6-BA to the regeneratingability of callus. Majority of the regenerated plants were diploids, but a fewtetraploids and mixoploids were observed.