Neurospora sp.の生産するプロテアーゼインヒビターの諸性質

概要

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For the purpose to obtain fungi which are capable of producing trypsin inhi-bitors, screening test was carried out. A strain, which belongs to the genus of Neurospora was obtained, and production of the inhibitor by the strain and some properties of the inhibitor produced were examined. Results obtained were summa-rized as follows : 1) The strain produced the inhibitor maximally, when it was cultivated with shaking in a medium containing 2.0% sucrose, 2.0% peptone, 1.0% yeast extracts, 0.1% KH₂PO₄ and 0.1% MgSO₄・7H₂O (pH 5.0) at 30 C for 7 days. 2) The inhibitor was partially purified with 80% acetone, 70% saturated ammonium sulfate and 50% ethanol. 3) Molecular weight of the inhibitor was speculated to be 10000 to 30000. The inhibitor was stable both in acid and neutral solutions, and unstable in an alkaline solution. Fifty percent of activity of the inhibitor was inactivated by boiling for 5 minutes in 0.1 M phosphate buffer, pH 7.0 and more than 90% was inactivated by boiling for 30 minutes. 4) The inhibitor inhibited Nagase, papain and Pronase as well as trypsin, but not a-chymotrypsin and pepsin. 5) The inhibitor obtained in this experiment was quite different from other protease inhibitors from microorganisms in their properties.
信州大学農学部の論文
1976-12-25