Genetic verification of induced gynogenesis and microsatellite–centromere mapping in the barfin flounder, Verasper moseri

概要

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Primer sets were newly developed for 34 polymorphic microsatellite loci in the barfin flounder Verasper moseri. Mendelian inheritance was confirmed by examining the genotypic segregation in 2 normal diploid full-sib families. All the 34 loci showed genotypic segregation according to the Mendelian manner of inheritance; in some cases, null alleles were assumed. The genotypes at 27 loci were also examined in 4 meiotic gynogenetic diploid lines produced by fertilizing eggs with UV-irradiated sperm, followed by inhibition of the second meiotic division by cold shock. The absence of paternal alleles verified the success of gynogenetic development in all 4 meiotic gynogenetic diploid lines; the proportion of heterozygous progeny of a heterozygous mother, i.e., the frequency of second division segregation (y), was used to estimate the map distance of each microsatellite locus in relation to the centromere. Marker-centromere distances were estimated to be in the range of 0 to approximately 50 centiMorgan (cM) under the assumption of complete interference. Using 8diagnostic loci located at the telomeric region of the chromosome, complete homozygosity was confirmed in 1 mitotic gynogenetic diploid line that was produced by suppressing the first cleavage via hydrostatic pressure shock.
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