Purine metabolic enzymes in lymphocytes. III. Effects of immunosuppressants on adenosine deaminase and purine nucleoside phosphorylase activities

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Mice were treated with a single injection of 6-mercaptopurine riboside (6MP-R), predonine or cyclophosphamide (CY), and the effects of these immunosuppressants on blastogenic responses to phytohemagglutinin P (PHA-P) or bacterial lipopoly saccharide (LPS) and on adenosine deaminase (ADA) or purine nucleoside phosphorylase (PNP) activities were studied with spleen lymphocytes. The retardation of blastogenic responses to both PHA-P and LPS were associated with the retardation of both intracellular ADA and PNP activities in 6MP-R-treated mouse spleen lymphocytes. Both PHA and LPS responses were suppressed in lymphocytes from mice treated with predonine, whereas no suppression of both ADA and PNP activities was observed. In mice treated with CY, LPS response of spleen lymphocytes was markedly suppressed but PHA response was almost the same with that of normal lymphocytes. PNP activity was retarded in lymphocytes of CY-treated mice, while ADA activity of them was rather enahnced. These results suggest that the suppression with 6MP-R may be corresponding to the inherited immunodeficiency with enzyme deficiency, and that the suppression with predonine may be corresponding to immunodeficiency which is not associated with enzyme deficiency.
1983-03-30