〈原著論文〉ニワトリ５日、７日および１０日胚の未分化生殖巣に存在する生殖細胞（ＧＧＣｓ）の移住能力に関する研究

概要

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In order to evaluate the migratory ability of the gonadal germ cells, embryonic gonads were collected from 5-, 7- and 10-day-old White Leghorn (WL) embryos and dispersed in medium containing 0.25% trypsin. Gonadal germ cells (GGCs) were collected and labeled with fluorescent dye (PKH-26). Approximately 150 GGCs were injected into the dorsal aorta of 2-day-old recipient embryos, which were then incubated for 5 days in a forced-air incubator. After incubation, gonads from recipient embryos were recovered, dispersed using 0.25% trypsin, and observed under a fluorescence microscope. Fluorescence-labeled GGCs were detected in the gonads of recipient embryos injected with GGCs from 5- and 7-day-old chick embryos, but not from 10-day-old chick embryos. These results indicate that GGCs retain the migratory ability for at least 7 days of incubation in vivo.
2004-03-31