J024022 厚みのある組織の凍結保存における生存率改善に向けた冷却速度の検討/([J02402]バイオにおける流れと熱・物質移動(2))

概要

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In order to be successful cryopreservation of thick tissue, understanding of the mechanisms of cell in cryopreservation and the surrounding environment is very important. In this study, by giving the freezing direction to the cell monolayer with controlled cell orientation to simulate the organization of thick, the effect on cell viability post-thaw changes in cooling rate at various sites of thick tissue. Method, human dermal fibroblasts were cultured to be oriented on the substrate 24 x 24mm, the test samples were frozen with direction from the bottom to the tops. This time, temperature changes during freezing was measured by a thermocouple attached to three sites, bottom, middle and upper. From the cooling rate obtained from the temperature of each sites in the samples and post-thaw cell viability, the faster the controlled cooling rate, the larger the width of the upper and lower cooling rate resulted in a strong non-linear cooling curve . Cell viability also tended to decrease the survival rate is not strong enough nonlinear. Therefore, the impact on post-thaw cell viability of the difference in the average cooling rate of thick parts of the organization is small, indicating the potential to mitigate the decrease in survival due to the nonlinearity of the cooling rate.
一般社団法人日本機械学会の論文
2011-09-11