205 磁性マイクロピラーによる細胞焦点接着部への振動刺激の印加手法の開発

概要

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This paper described development of a micro device which applies mechanical vibration stimulation to focal adhesions of a cell. In recent years, evidence has been growing that focal adhesions act as mechanosensors of cells which convert mechanical force into biomechanical signaling. However, there are no effective micro devices which can directly apply mechanical stimulation to each focal adhesion. Here we develop a micropillar substrate embedding micron-sized magnetic particles and enabling the micropillars to be deflected by external magnetic field. The combination of long and short micropillars produces the difference of deflection between them and enables the micropillars to apply deformation to cells. The long pillars were periodically deflected at the amplitude of approximately 1.4 μm whereas many of short pillars were not deflected. The cell culture on the magnetic micropillar substrate revealed that this device can apply periodic deformation to focal adhesions of a cell. These findings indicate that the present micro device can be used for investigating mechanosensing systems of a cell.
一般社団法人日本機械学会の論文
2011-09-05