203 培養骨芽細胞に及ぼす機械的振動の印加時間の影響

概要

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According to previous studies, when some mechanical vibration is operated to osteoblast-like MC3T3-E1 cell, proliferation of the cell depends on the frequency and acceleration amplitude of applied vibration. But there are lots of unsolved mechanism between biomechanical stimulation and cellular behavior. In this study, the attention was focused to the effects of mechanical vibrating term on cultured osteoblasts. Cells, MC3T3-E1, were seeded in three culture plates. After seeding, the three plates were left still in a CO_2 incubator. And two plates were excited by electromagnetic exciter at frequency of 12.5Hz and acceleration amplitude of 0.5G in the incubator. Non-stop vibration was applied to one of two plates, which was called "group 1". A regular-term vibration was given to another plate, called "group 2". One plate was set still without vibration, called "control group". Cell density was measured and the medium in plates was changed every 3days. The difference between control group and vibration group was investigated by the growth curve.
一般社団法人日本機械学会の論文
2011-09-05