OS1001 β-TCP/コラーゲン複合系scaffoldを用いた間葉系幹細胞の培養に関する基礎的研究(OS10-1 医療用無機/有機複合材料の力学特性,OS-10 医療用高分子系複合材料の構造と力学特性)

概要

論文の詳細を見る
Recent years, porous composite scaffolds consisting of natural polymer and bioceramics have been considered to be used in bone tissue engineering. Collagen guarantees excellent biological conditions such as stimulation of generation and differentiation of cells. A typical bioactive ceramics, β-TCP, has good oseteoconductivity, cellular adhesion, accelerated differentiation and mechanical property. In this study, β-TCP/collagen composite scaffolds were developed by the freeze-drying technique and rat bone marrow mesenchymal stem cells were cultured in the scaffolds up to 28 days in order to assess the effect of cell growth on the mechanical behavior. The cell number and ALP activity were evaluated using a spectrophotometric plate reader. Compression tests were also conducted periodically by using a conventional testing machine to evaluate the elastic modulus. The increasing behavior of cell number and ALP activity with the composite scaffolds was much better than that in pure collagen scaffold. The compressive modulus also increased with increase of culture period. These results clearly indicate that β-TCP is very effective to enhance the cell growth behavior and the mechanical property.
一般社団法人日本機械学会の論文
2011-07-16